Résumé
Human cytomegalovirus glycoprotein complex Ⅱ (gC Ⅱ ) consists of two glycoproteins, gM and gN. Although gC Ⅱ specific IgG purified from HCMV positive patient sera can neutralize HCMV, there has been no report on the generation of virus-neutralizing antibodies by immunizing with one epitope of gM. The epitope, termed MAD, was screened from random phage peptide library by subtractive strategy. The peptide sequence of MAD was highly homologous with 32~38 amino acids of HCMV gM. Mice immunized with MAD coupled with keyhole limpet hemocyanin (KLH) could produce specific antibodies against MAD, and the antibodies obtained could bind not only native HCMV particles, but also the recombinant gM30～78 peptide. ELISA analysis results showed that MAD could specifically bind HCMV-positive human serum samples. Virus-neutralizing assay results demonstrated that the antibodies against MAD could inhibit HCMV strain AD169 entering the human embryonic lung cells. The results suggested that MAD could be used as a new potential protective antigen in the development of HCMV vaccine.
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Objective To investigate the status and influencing factors of knowledge,attitude and practice(KAP) about AIDS among the migrant miners in a nonferrous metal company of Anhui Province and to provide evidence for constituting strategies and measures for AIDS health education and behavioural intervention.Methods 303 migrant miners in a nonferrous metal company were surveyed.Univariate analysis,Logistic regression and correlation analysis were used to explore their influencing factors.Results The general rate of awareness of AIDS was 87.9%,and the main factor affecting miners knowledge level of AIDS was their educational level.Only 55.1% of migrant miners thought people with AIDS should not be isolated;50.8% of them would like to contact people with HIV;38.0% did not object to extramarital sex.There were 32(10.6%) migrant miners admitting having had extramarital sex in the recent three months,but 11(34.4%) admitted never having used condoms,only 7(21.9%) having used each time.Multivariate analysis identified the influencing factors for condom utilization including age and attitude to people with HIV/AIDS.Conclusions ①The migrant miners have sufficient knowledge about AIDS.②The traditional sexual beliefs are changing,and an increasing number of people are tolerant to extramarital sex.Consequently,it is likely that extramarital sex might rise.③The promotion of condom use should be strengthened.
Résumé
<p><b>OBJECTIVE</b>To measure the immunogenicity of a synthetic peptide of glucosyltransferase (GTF) for designing synthetic peptide-based vaccine of dental caries.</p><p><b>METHODS</b>A fusion 27-mer peptide, containing the conserved regions within catalytic and glucan-binding domains of GTF, was synthesized. Serum antibodies to the synthetic peptide were determined by ELISA method. Inhibitions of both GTF activity and S. mutans adherence were detected for the functions of antisera.</p><p><b>RESULTS</b>The sequence of fusion peptide vaccine was ANDVDNSNPVVQAEQLYFRANGVQVKG. The spleen weights of immunized mice were heavier than the control ones. Specific antibodies were effectually elicited. The immune sera not only inhibited GTF enzymatic activity but also inhibited the vitro adherence of S. mutans.</p><p><b>CONCLUSIONS</b>The peptide vaccine which involves antibody-mediated inhibition of the catalytic and the glucan-binding activities of GTF may be valuable for controlling the dental caries.</p>