Résumé
<p><b>OBJECTIVE</b>To explore the effect of Herba Epimedii flavone (HEF) on the osteoblast metabolism in vitro.</p><p><b>METHOD</b>Osteoblast were obtained from new born rat calvaria by digestive enzymes. MTF, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.</p><p><b>RESULT</b>It was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts (P < 0.01, P < 0.05).</p><p><b>CONCLUSION</b>HEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.</p>
Sujets)
Animaux , Rats , Phosphatase alcaline , Métabolisme , Animaux nouveau-nés , Protéines de transport , Génétique , Prolifération cellulaire , Cellules cultivées , Epimedium , Chimie , Flavones , Pharmacologie , Glycoprotéines , Génétique , Glycoprotéines membranaires , Génétique , Ostéoblastes , Biologie cellulaire , Métabolisme , Ostéoprotégérine , Plantes médicinales , Chimie , Ligand de RANK , ARN messager , Génétique , Rat Sprague-Dawley , Récepteurs cytoplasmiques et nucléaires , Génétique , Récepteurs aux facteurs de nécrose tumorale , GénétiqueRésumé
<p><b>OBJECTIVE</b>To observe the effect of puerarin on cell proliferation, differentiation, maturation and mineralization in cultured rat osteoblasts.</p><p><b>METHOD</b>Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.</p><p><b>RESULT</b>It was found that puerarin had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).</p><p><b>CONCLUSION</b>Puerarin can promote proliferation, differentiation, maturation and mineralization of the osteoblasts in vitro.</p>