Résumé
Objective To observe the clinical efficacy of Dane Fukang Jiangao combined with gonadotropin-releasing hormone analogue(GnRH-a)in the treatment of endometriosis(EMT)after laparoscopic surgery. Methods A total of 120 EMT patients treated with GnRH-a after laparoscopic surgery were randomly divided into combine treatment group(n=60) and control group (n=60). In the control group, GnRH-a 3.75 mg was injected subcutaneously on the first day of menstruation and injected once every 28 days. The combine treatment group was given Dane Fukang Jiangao with oral administration 10 g per time, twice a day for 6 months. The changes of estradiol (E2), CA125 and visual analogue scale (VAS)were observed before and after treatment.Kupperman score was used to assess the degree of menopausal symptoms during treatment and follow-up records of recurrence. Results Before treatment, there were no significant differences in the serum levels of E2 and CA125 and VAS between both groups.After treatment,the serum levels of E2 and CA125 and VAS were significantly improved in both groups. And the serum level of E2 was significantly higher in combine treatment group than that of control group (P<0.05). The VAS and the serum level of CA125 were significantly lower than those of control group(P<0.05).Moreover,after treatment the Kuppermann score was significantly better in combine treatment group than that of control group (P<0.05). There was no significant difference in the recurrence rate in 6 months between the combine treatment group (21.7%) and the control group (38.3%, P>0.05). Conclusion Dane Fukang Jiangao in combination with GnRH-a can effectively prevent E2 from being too low,reduce the CA125 level after EMT surgery,relieve dysmenorrhea and menopausal symptoms,and prevent recurrence.
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As a rapidly developing area in biomechanics, mechanics of biological materials aims to investigate the fundamental relationship between functions, properties, structures, and compositions of various natural biological materials at different length scales and the underlying physical mechanisms through experimental research and theoretical analysis, and to provide inspirations from the splendid living world for the biomimetic design of new materials. In this paper, some recent achievements, mainly made by Chinese scholars in this field, are briefly reviewed, including the stiffening and toughening mechanisms of such biological composites as nacres and horns, the relation between the surface wetting behavior and the micro/nanostructures, and some relevant biomimetic applications as well. The contents of seven invited papers published in this special issue are also introduced.
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<p><b>OBJECTIVE</b>To observe and compare the luciferase activities of different length segments of human dentin matrix protein 1 promoter in human dental pulp stem cells (HDPSC), osteoblasts (OC) and Hela cells.</p><p><b>METHODS</b>The differentlength desired DNA segments were obtained from 2 195 bp Dmp1 promoter cloned by PCR method. The amplified promoter segments with different length were cloned into luciferase report gene vector pGL3-Basic, the correct orientation of those inserts was verified by cutting with two different restrict enzymes. The luciferase activity was observed after different pGL3-PDmp1 vectors were transfected transiently into those three different-type cells.</p><p><b>RESULTS</b>6 Dmp1 promoter segments with different-length were obtained successfully, and luciferase report gene vectors with different promoter segments were successfully constructed after identified by restriction enzymes cutting. They had different luciferase activities when they were transfected transiently into HDPSC, and the region of -505(-)-193 bp and -935(-)-505 bp could be regarded as the specific promoters of Dmp1 promoter for HDPSC and OC respectively, which could include the basic regulatory elements.</p><p><b>CONCLUSION</b>The correct clone of the upstream of human Dmp1 promoter segments with different length had been obtained, and they had strong luciferase activities in HDPSC and OC, but very low in Hela cell. These results will make an important basis for studying mineralized tissue-specific transcriptional regulation mechanisms of Dmp1.</p>