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OBJECTIVE To study the distribution and drug resistance of meticillin-resistante coagulase negative Staphylococcus(MRCNS) in neonate septicemia for rational use of antibiotics in clinic. METHODS The MRCNS isolates were detected by BD120 and ATB.Susceptibility test to 12 antibiotics was performed through disk diffusion test. RESULTS Totally 262 strains coagulase negative Staphylococcus(CNS) was isolated from 344 S.aureus strains.Among 262 CNS,there were 130 S.epidermidis strains(49.6%),94 S.hemolyticus strains(35.9%),21 S.hominis(8.01%),and 17 other kinds of Staphyloccus strains(6.49%).The isolated ratio of MRCNS was 65.0%(170/262).Susceptibility test showed that the MRCNS were multiple resistant. CONCLUSIONS CNS occupys the first place in infection of neonatal blood.The isolated ratio of MRCNS is high and shows multi-drug resistance.Vancomycin and some other glycopeptide antibiotics are the first choice for MRCNS infections.
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OBJECTIVE To investigate the evolution of drug-resistance of Staphylococcus aureus in children in Hubei tertiary level hospitals during four years and provide the evidence for clinically reasonable use of antibiotics.METHODS The summary data of drug sensitivity in clinical isolates of meticillin-resistant S.aureus(MRSA) and meticillin-sensitive S.aureus(MSSA) in children in Hubei tertiary level hospitals from 2004 to 2007 were investigated retrospectively and the results were analyzed statistically.RESULTS The tendency of infections with MRSA upgraded yearly during four years(5.7%→10.7%→40.8%→46.9%);the resistance rates of MRSA to various antibiotics were all higher than that of MSSA.The vancomycin-resistant S.aureus had not been detected.CONCLUSIONS The drug-resistance status of SA in Hubei is still very serious.We should adopt effective measurement to control the occurring and epidemic outbreak in combination with children physiological characteristic.
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OBJECTIVE To study the distribution of drug-resistant gene of meticillin-resistant coagulase negative Staphylococcus(MRCNS) in children septicemia.METHODS The total MRCNS isolates were 40,and whether in there harbored genes mec,erm and qac was studied.RESULTS Among 40 CNS strains,in there harbored genes mec,erm,and qac were 38(95.0%),30(75.0%) and 18(45.0%),respectively.CONCLUSIONS MRCNS in children septicemia where harbor drug resistance genes is very serious,so we should pay great heed to its effective control.
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OBJECTIVE Investigating the distribution and drug-resistance of pathogenic bacteria in urinary tract infecion for children in Wuhan region to provide the evidence of diagnoses and treatment for clinician.METHODS The positive results of urine culture of 405 children with urinary tract infections from Jul 2002 to Dec 2005 were analyzed.All of the isolates were tested by Kirby-Bauer susceptibility method.RESULTS Among 405 pathogens,the majority was Escherichia coli which occupied 36.7%;then Enterococcus accounted for 29.4%.Totally 405 pathogens all showed the higher drug resistance,while antibacterial antivities of imipenem,amikacin and furantoin to E.coli and Klebsiella pneumoniae and of glycopeptides,high concentration of streptomycin and furantoin to Enterococcus were preserved well.CONCLUSIONS The effective measurements and reasonable use of antibiotics should be taken to hold back the occurrence and prevalence of urinary tract infecion for children.
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OBJECTIVE To investigate dynamically antibiotic resistance changing of Escherichia coli in Hubei province and provide the reference for clinical rational application of antibiotics.METHODS The WHONET4-5 software was used to analyze the antibiotic resistant rates of 2901 strains of E.coli isolated from all kinds of clinical specimen of children from 2003 to 2006 in the third grade hospitals of Hubei province.RESULTS Drug-resistance rate of E.coli in children group was lower than that in adult group.Except for 100.0% susceptibility to carbopenems such as imipenem and Meropenem,in the four years,drug-resistance of the other antibiotics showed ascending tendency.Ampicillin showed the highest drug-resistance rate(84%-90%).The lower(3%-25%) were amikacin,cefoxitin,ceftazidime,cefepime.CONCLUSIONS Antibiotic resistance of E.coli in children in Hubei province showed the ascending tendency.We should take effective measurement to control the spread and prevalence of the drug-resistant bacteria.
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OBJECTIVE To approach biosafety risks in laboratory department of children′s hospital to formulate the managemen strategy.METHODS The existing biosafety risk in laboratory department of children′s hospital was investigated and analyzed.RESULTS A series of problems such as personnel′s biosafety consciousness,work environment,protection equipment,a medical orderly′s training,etc were existing.CONCLUSIONS Biosafety regulations should be executed strictly in children′s hospital,and biosafety management should be highly paid attention to and consummated.