Advances in innate immune responses induced by Mycoplasma hyopneumoniae infection / 生物工程学报

Mycoplasma hyopneumoniae is the pathogen causing swine mycoplasmal pneumonia. The lack of well-established animal models of M. hyopneumoniae infection has delayed the progress of M. hyopneumoniae-related anti-infection immunity studies. This paper reviews the inflammatory response, the recognition of M. hyopneumoniae by the innate immune system, and the role of innate immune cells, complement system, antimicrobial peptides, autophagy, and apoptosis in M. hyopneumoniae infection. The aim was to elucidate the important roles played by the components of the innate immune system in the control of M. hyopneumoniae infection, and prospect key research directions of innate immune response of M. hyopneumoniae infection in the future.

Fighting between bacteria and autophagy--death or rebirth / 生物工程学报

Autophagy is a highly conserved degradation process that targets cytoplasmic components, maintains metabolic stability in cells, and combates infection with various pathogenic bacteria. Autophagy can help body to eliminate invading pathogens; however, some bacteria have evolved multiple strategies to interfere with the autophagy signaling pathway or inhibit the fusion of autophagosomes with lysosomes to form autolysosomes to escape autophagic degradation, and even use autophagy to promote their growth and proliferation. This review discusses the newest progress in the relationship between pathogens and autophagy of host cell, and the role of autophagy in bacterial infection. We hope that this review provides useful knowledge for the research on autophagy caused by pathogenic infection.

Development of an ELISA for identification of immunodominant protein antigens of Mycoplasma hyopneumoniae / 生物工程学报

We developed a method to identify serological humoral immunodominant proteinic antigen of Mycoplasma hyopneumoniae (Mhp). After constructing the recombinant plasmid pGEX-6P-1-mhp366 and transforming it into Escherichia coli BL21(DE3), the recombinant GST-Mhp366 protein was expressed successfully. The lysates of the recombinant GST-Mhp366 and genetic engineering GST were added into glutathione coated plates and reacted with 17 positive sera or 13 negative sera. Meanwhile, the optimization of experimental conditions, including coated antigen, blocking buffer, dilutions of sera and second antibody were determined. The optimal concentration of the coated antigen was the original bacteria lysates without dilution, and the optimal blocking buffer contained 10% FBS and 2.5% skim milk in PBS. Besides, the working concentration of serum samples and the HRP-tagged rabbit anti-pig IgG secondary antibody were 1:500 and 1:40 000, respectively. Thus, an indirect ELISA was established for identification of immunodominant protein antigens of Mhp. Meanwhile, this method was confirmed by the identified serological humoral immunodominant proteinic antigen Mhp156 and Mhp364. This method can be used for identification of the candidate vaccine antigens on a genome-wide scale. Furthermore, it can lay the foundation for identifying the candidate vaccine antigens through colostra and the nasal mucosal secretions.

Review on Experience of CUI Shu-sheng's Practice in Treating Insomnia / 中国中医药信息杂志

Insomnia is the important factor affecting people's lives. The long-term lacking of sleep can not only affects people's daily life, but also can lead to a variety of mental illness. With the development of society, the life and working styles of people have changed a lot, and the number of patients with insomnia is increasing. However, the main therapy of insomnia is the Western medicine with calming and sedative efficacy. This kind of medicine has side effects, and long-term application can cause resistance and addiction. This article introduced the fourth batch of 100 old TCM doctors Professor CUI Shu-sheng's profound understanding of insomnia etiology and pathogenesis. Combined with the function features of Tuina, he summarized the Ten Methods of head massage. The Ten Methods of head massage has the characteristics which are light but not float, heavy but not hurt, and gentle and strong. The main operation places are head and face; thus, it can relieve the scalp and dredge qi and blood of head and face. Because the doctor can guide patients to calm down and focus on their public region and soothe their body, it has the effects of harmonizing yin and yang, calming, and soothing.