RÉSUMÉ
BACKGROUND:Many studies concerning influence of traditional Chinese medicine on animal movement capacity have been conducted.However,studies on the improvement of exercise capacity by traditional Chinese medicine are few,especially comparisons among traditional Chinese medicineOBJECTIVE:To explore the influence of different traditional Chinese medicine preparations on the exercise capacity in mice to screen compound preparations with precise effects.METHOD:A total of 80 8-week-old pure male Kunming mice were randomly assigned to 8 groups(n=10):control and compound 1-7 groups.There were no significant differences in body mass of all groups The compound 1 comprised Xuejie and Shexiang;compound 2 comprised Qingui,Baizhu and Baishao;compound 3 comprised Baizhu,Baizhi and Sanqi;compound 4 comprised Baizhi,Ruxiang,Danggui and Radix glycryrrhizae;compound 5 comprised Guiwei;compound 6 comprised Sichuan Honghua and Danshen;compound 7 comprised Danggui and Shexiang.Of the 7 prescriptions,compound 1-5 were empirical formula,compound 6 was common medicine used during training,and compound 7 was prescription of traditional Chinese medicine The 7 compound groupswere respectivelytreatedwith 1.17,0.78,0.78,1.17,7.02,5.53,and 11.70 g/kg per day for 4 weeks,and control group was intragastrically infused with normal saline.From day 4 to 6,load-free swimming training was performed 20 minutes a day for 3 days,followed by swimming for 6 days a week after 1 day of rest.The intensity was gradually increased frOm 30 minutes per day by 10 minutes per day for 2 hours,followed by loading swimming with load of 2%of the body mass,increased by 1%per day until the final experiment.During the entire procedure,a great intensity swimming training was performed every week to make the mice exhausted.Mice training and serum creatine kinase and lactate dehydrogenase 4 weeks following treatment were observed.RESULTS AND CONCLUSION:A total of 80 mice were included in the final analysis The compounds showed little influence on mice body mass;the compound 2 and compound 6 groups had longest exhaustive time All compounds inhibited serum creatine kinase activity following exercise,in particular compound 6 and 2(P＜0.05).Compared with control group,the serum lactate dehydrogenase activity of each compound group was decreased,in particular,compound 2 and 6 groups showed significant differences(P＜0.05).Compound 2 and 6 significantly enhanced the exercise capacity of mice and delayed injury of body.The mechanism may highly relate to components in the compounds,Danggui,Sichuan Honghua and Danshen.
RÉSUMÉ
BACKGROUND: Exercise-induced skeletal muscle apoptosis has been referred to as the current focus of sports medicine, and the application of stem cells in athletic injury rehabilitation and prevention has been reported. However, the role of stem cells in cell apoptosis remains unclear.OBJECTIVE: To summarize the effect and mechanism of stem cells in preventing exercise-induced skeletal muscle apoptosis to provide references for scientific sports training and physical activity.METHODS: A computer-based online search of PubMed (1991-01/2009-10) and CNKI (1994-01/2009-10) was performed for related articles with the keywords ""Exercise Training, Sports, Skeletal Muscle, Apoptosis" in English and "stem cells, exercise, skeletal muscle, apoptosis," in Chinese. Inclusion criteria: ① studies on stem cells and their apoptosis in skeletal muscle cells; ②articles in the same field published recently or in the authoritative journals. Exclusion criteria: ① Repetitive articles; ② Meta analysis.RESULTS AND CONCLUSION: A total of 360 articles were collected from the summary of literature through the stem cells and its application in the field of sports medicine research, and its skeletal muscle apoptosis in the changes and development trend of the application. Finally, 31 articles were included, including 21 reviews and 10 clinical or experimental studies. High-intensity exercise can cause apoptosis in skeletal muscle cells, while the use of stem cell technology can prevent apoptosis, to a certain extents by regulating Bcl-2 and Bax protein expression, thereby promoting the early recovery of skeletal muscle.
RÉSUMÉ
BACKGROUND: Exercise-induced myocardial cell apoptosis and signal transduction and regulation mechanisms of sports medicine research have become an important issue, but remain poorly understood. Studies concerning the protective effect of bone marrow-derived mesenchymal stem cells (MSCs) to the exercise-induced myocardial cell apoptosis are rarely conducted.OBJECTIVE: To analyze the protective effect of MSCs on myocardial apoptosis and the exercise-induced myocardial cell apoptosis, so as to explore the pathological cause of exercise-induced myocardial apoptosis and the protective effects of MSCs on myocardial cell apoptosis induced by hypoxia, ischemia, and oxidative stress.METHODS: A computer-based online search of articles was performed in Medline database (1994-01/2009-09) with "Mesenchymal stem cells, Excessive exercise, Cardiomyocyte, Apoptosis" as key words and Chongqing PubMed Result NCBI database (1994-01/2009-09), Tsinghua Tong Fang database (1994-01/2009-06), with "bone marrow mesenchymal stem cells, over-training, myocardial cells, apoptosis" as key words. RESULTS AND CONCLUSION: A total of 365 articles on stem cells and exercise-induced myocardial apoptosis were collected, including 120 Chinese and 245 English. Outdate, repetitive and similar studies were excluded, and 68 were included. MSCs display a protection to myocardial cell apoptosis induced by hypoxia, ischemia and oxidative stress caused by high-intensity or overload exercise training, thereby contributing to the improvement of heart function and early rehabilitation of exercise-induced myocardial tissue diseases.