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Objective To investigate the preventive and therapeutic effects of 1,25-(OH) 2D3 on airway remodeling in asthmatic rats,and to explore the effect of 1,25-(OH) 2D3 on the expression of transforming growth factor-β 1 (TGF-β 1) and the related mechanism between them.Methods Seventy male Sprague Dawley (SD) rats were randomly divided into seven groups:normal control group (group A,n =10),asthmatic group (group B,n =10),dexamethasone group (group C,n =10),1,25-(OH)2D3 2 μg/(kg·d) group (group D,n=10),1,25-(OH)2D3 4 μg/(kg · d) group (group E,n =10),1,25-(OH)2D3 8 μg/(kg · d) group (group F,n =10),1,25(OH)2D3 16 μg/(kg · d) group (group G,n=10).Rats were sensitized and challenged with ovalbumin (OVA) to establish the asthmatic model in B,C,D,E,F,G group respectively.Rats in group B and group C were fed with normal saline 10 ml/(kg · d) and dexamethasone 2 mg/(kg · d) before atomization.While group D-G was 1,25-(OH)2D3 intervention group,rats were fed with vitamin D 2,4,8,16 μg/(kg · d) before atomization.The number of total and different cells of the bronchoalveolar lavage fluid (BALF) from the 7 groups was counted for analysis;The change of the thickness,the area of airway wall and the number of ASMC nucleus and pulmonary tissue changes were observed by microscope.The protein and mRNA expression of TGF-β1 in the lung tissue was measured by immunochemistry and real-time polymerase chain reaction (RT-PCR).Results (1) The airway remodeling and pulmonary inflammatory exudation in the B group were significantly heavier than those in the A,C and G groups (P ＜0.01);and the difference between groups of different doses of vitamin D [D group (minimum dose) and G group(the maximum dose)] was statistically significant (P ＜ 0.01).(2) The results of TGF-β1 mRNA and protein levels in rats of each group showed that the expression level of TGF-β1 mRNA and integrated option density (IOD) value in A group were significantly lower than those in other groups (P ＜0.01);the expression level of TGF-β1 mRNA and IOD value in B group was higher than the other groups (P ＜0.01);Compared with the B group,the expression level of TGF-β1 mRNA and IOD value in G group (large dose vitamin D group) was decreased,with statistically difference.Conclusions In the rat model of asthma,1,25-(OH) 2D3 may alleviate airway remodeling by regulating the expression of TGF-beta 1 and play its opposite biological effect in the prevention and treatment of asthma.
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ObjectiveTo explore the early diagnosis and effective treatment of redundant colon,and to reduce the misdiagnosis and shorten the medical treatment time before the diagnosis.MethodsClinical data of twentyseven patients with redundant colon from February 2005 to December 2011 were retrospectively analyzed in General Surgery Department of 117th and 322th People's Liberation Army Hospital.ResultsThe clinical symptoms of 27 patients nainly as early recurrent intractable constipation,bloating,abdominal pain,weight loss and other symptoms,were likely to be in a misdiagnosis.In addition to three patients with redundant sigmoid colon concurrent reverse came to hospital emergency with surgery,twenty-four cases' symptoms persisted and came to many hospitals with medical treatment up to 32 years,diagnosed by the out-patient barium enema.After surgical resection disease bowel,7-11 months follow-up,patients abdominal distension,abdominal pain,constipation,weight loss and other systemic unwell symptoms disappeared.ConclusionThis disease is rare,we must raise the medical staff's awareness of this disease.X-ray examination with barium enema is the best way to diagnose this disease.After diagnosis,surgery is the most effective treatment.
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AIM: To study the effects of arsenic trioxide (As_2O_3) on mouse airway fibroblast (FB) proliferation and expression of c-myc and c-sis in cultured FB. METHODS: The cultured FB was divided into seven groups. NS, dexamethasone (1.0 ?mol/L) and As_2O_3 at concentrations of 0.25, 0.5, 1.0, 2.0, 4.0 ?mol/L were instilled respectively into cultured FB. The effects of different concentration of As_2O_3 and dexamethasone on cell growth were observed at 1, 3, 5 and 7 day. The changes of cell cycle and the positive expression rate of c-myc and c-sis were examined by flow cytometry (FCM). RESULTS: Various concentrations of As_2O_3 significantly inhibited the proliferation of FB in vitro and showed a dose-dependent and time-dependent tendency. Data from FCM indicated that G_1-phase cell percentage increased and G_2/M-phase cell percentage decreased with the increase in As_2O_3 concentrations. The expression of c-myc and c-sis was significantly inhibited by As_2O_3 (2.0, 4.0 ?mol/L). CONCLUSION: As_2O_3 inhibits FB proliferation by downregulating the expression of c-myc and c-sis.