Cellular immune response in Giardiasis

In this study, adult persons attending the outpatients clinics of Tropical Medicine in Mansoura University, Banha Branch, Zagazig University as well as in the Clinical Pathology of Ain-Shams Specialized Hospitals were subjected to stool examination using Kato thick smear and repeated concentration technique. There was a statistically significant difference between all groups of giardiasis patients regarding the grade of CD4 lymphocyte infiltration, being more marked in the symptomatic group. The prevalence of flatulence, anorexia and vomiting was more frequent in patients with heavy CD4 lymphocyte infiltration in duodenum. A high statistical significant increase was observed in the mean OD values of anti-giardia duodenal secretory IgA in patients with marked CD4 infiltration in duodenum. But, a statistical insignificant difference was found in the mean OD values of anti-giardia total serum Ig in patients with different grades of CD4 infiltration in the symptomatic group. There was a statistically significant increase in the mean OD values of anti- giardia total serum Ig in patients with marked intraepithelial CD8 lymphocyte infiltration in the duodenum in the asymptomatic group. There was a statistically insignificant difference in the mean OD values of anti-giardia total serum Ig in patients with different grades of intraepithelial CD8 infiltration in the symptomatic group. Also, there was statistically significant increase in the mean OD values of anti-giardia total serum Ig in patients with marked intraepithelial CD8 lymphocyte infiltration in the duodenum regarding the immunohistochemical staining of giardia antigen in duodenal biopsies

endoparasites of sheep and goats, and shepherd in north Sinai governorate, Egypt

The examination of sheep and goats in four areas in North Sinai revealed an overall infection of 12.70% with Fasciola species, 11.8% with Paramphistomum cerve, 12.80% with Moniezia expansa, 47.11% with Trichostrongylus colubriformis, 4.59% Trichuris ovis and 44.85% Coccidia. The highly infected sheep and goats were diagnosed in El- Hassanah Center [90.49%], followed by El-Arish City [87.31%], then Rafah City [74.39%] and lastly Bir El-Abd [54.71%]. The elevation of the overall percentage of infection in the four areas was due to Coccidia infection, as this parasite was showed a concomitant infection in nearly all animals. On the other hand, the examination of 15 of the shepherds revealed Ascaris lumbricoides in 5, T. colubriformis in 3, Fasciola in one, Giardia lamblia in 10 and Coccidia in 3. Double and rarely triple infection was seen. The correlation between parasitic infections in sheep and goats as well as their shepherd was discussed