Résumé
CD24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. Our aim was to clarify the correlation between CD24 expression and cAMPxGMP ratio in murine colorectal cancer cell line [CT26] after using cholera toxin. The CT26 cells were cultured in flasks for assaying cAMP and cGMP by ELISA kits; also the cells were cultured in flasks for assaying cytoplasmic and membranous CD24 expression. The Real-Time PCR was done for cDNA that was synthesized from CT26 cells' mRNA. Also, expression CD24 marker of cells was determined by Anti-CD24 antibody and Goat Anti-Rabbit IgG-FITC [flow cytometry]. The cholera toxin increases the cAMPrcGMP ratio and influenced the cytoplasmic and membrane CD24 expression level. Cholera toxin increased cAMP. After increasing cAMP/cGMP ratio, cytoplasmic and membranous CD24 expression showed decline