Sperm morphology does not influence fertilization rate in vitro / 中华男科学杂志

<p><b>OBJECTIVE</b>To determine the correlation of the rate of morphologically normal sperm, sperm head defect, teratozoosperm index (TZI) and sperm deformity index (SDI) with the fertilization outcome of IVF and intracytoplasmic sperm injection (ICSI).</p><p><b>METHODS</b>We detected normal sperm morphology, sperm head defect, TZI and SDI by Krüger's strict criteria and compared them with the rate of fertilization.</p><p><b>RESULTS</b>In either IVF or ICSI, the fertilization rate did not show any correlation with the normal sperm morphology rate, the head defect rate, TZI and SDI, nor any statistic differences were found in the rates of fertilization, clinical pregnancy and quality embryos either between the SDI > 1.6 and the SDI < 1.6 groups (P > 0.05) or between the normal sperm morphology rate > 15% and < 15% groups (P > 0.05).</p><p><b>CONCLUSION</b>Sperm morphology has no predictive value for the rate of fertilization in vitro.</p>

Impregnate occasion for male renal transplant recipients / 中华男科学杂志

<p><b>OBJECTIVE</b>To explore the impregnate occasion for male renal transplant recipients.</p><p><b>METHODS</b>Twenty-six male renal transplant recipients were divided into 3 groups according to the post-transplantation time and the administered dose of Cyclosporine A: 7 in Group A (less than 6 months after renal transplantation and at the dose of 4.1-6 mg/[kg x d]), 11 in Group B (6-24 months and 2.1-4 mg/[kg x d]) and 8 in Group C (longer than 24 months and 1.3-2 mg/[kg x d]). The semen of the patients were detected and compared with those of 12 normal volunteers.</p><p><b>RESULTS</b>Statistically significant differences were observed in sperm motility and sperm head deformity between Group A and C (P < 0.05), but not in pH value and sperm volume, vitality and concentration among the 3 groups (P > 0.05).</p><p><b>CONCLUSION</b>For male renal transplant recipients, 2 years or longer after the transplantation is the most suitable time for impregnation.</p>

Morphological characteristics of spermatozoa before and after renal transplantation / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To investigate the changes of the spermatozoa ultrastructures before and after renal transplantation in uremic patients.</p><p><b>METHODS</b>The sperm of five uremic patients before and after transplantation and four healthy volunteers were collected and examined by scanning electron microscopy.</p><p><b>RESULTS</b>Abnormal spermatozoa were found in patients pre-transplantation; abnormalities included deletion of the acrosome, absence of the postacrosomal and postnuclear ring, dumbbell-like changes of the head, tail curling, and absence of the mitochondrial sheath in the mid-segment. After renal transplantation, most of the spermatozoa became normal.</p><p><b>CONCLUSION</b>There are many abnormalities with regard to the appearance and structure of the head, acrosome, mitochondria and tail of the spermatozoa in uremic patients. The majority of the spermatozoa returned to normal after renal transplantation, but a few still presented some abnormalities possibly relating to the administration of immunosuppressants.</p>

Effects of different segments of acupuncture serum on eosinophil counts in the rat with eosinophilia / 中国针灸

<p><b>OBJECTIVE</b>To further analyze and identify effective components of anti-asthma in acupuncture serum.</p><p><b>METHODS</b>Changes of eosinophils in the peripheral blood of rats with eosinophilia were observed for 10 days after intravenous injection of the different segments of serum (serum: normal saline = 1:20, 2.5 mL/kg, from the first day of the model establishment, for 3 consecutive days).</p><p><b>RESULTS</b>After intravenous injection of different segments of serum, the eosinophil counts in the peripheral blood decreased significantly from the 3rd day as compared with those of the model group (P<0.05).</p><p><b>CONCLUSION</b>The effective components of acupuncture serum from asthmatic rats treated by acupuncture for eosinophils are not a single component, and acupuncture stimulation may produce many kinds of components of anti-asthma.</p>

Effects of different dosages of cyclosporine A on the semen parameters of renal transplant patients / 中华男科学杂志

<p><b>OBJECTIVES</b>To evaluate the effects of different dosages of cyclosporine A (CsA) on the main semen parameters and sperm morphology of the patients after renal transplantation.</p><p><b>METHODS</b>The semen of 18 patients after renal transplantation treated with different dosages of CsA was analyzed and the semen parameters and sperm morphology were compared with those of 12 normal volunteers.</p><p><b>RESULTS</b>There was not significant difference between the main parameters of the patients treated with 1.5-3.0 mg.Kg-1.d-1 of CsA and 3.1-5.5 mg.Kg-1.d-1 of CsA and those of the volunteers (P > 0.05), but the rate of normal sperm morphology was significantly different between the two groups(P < 0.05).</p><p><b>CONCLUSIONS</b>Different therapeutic dosages of CsA did not have any effect on most of the semen parameters of the patients after renal transplantation, but did affect the sperm morphology.</p>

Expression and purification of recombinant huwentoxin-I in Pichia pastoris / 生物工程学报

HWTX-I is a peptide neurotoxin purified from the crude venom of the Chinese bird Spider Selenocosmia Huwena, which has analyesic activity. rHWTX-I expressed by P. pastoris and secreted to culture supernatant was first precipitated by (NH4)2SO4, then it was isolated and desalted by ultrofiltration following by ion exchange chromatography of CM column, after reverse phase HPLC of C18 column and vacuum drying, the pure HWTX-I protein was obtained which was proved to be recombinant HWTX-I by Tricine SDS-PAGE, MALDI-TOF mass spectrometry, amino acid composition analysis, the N-terminal amino acid sequence and its biological activity. The final yield of the purified HWTX-I was about 80 mg/L accounting for 23.6% of its total secretory proteins.