Résumé
A capillary feeding technique was developed to provide an easy rapid method to asses L3 larvae transmission by infected Cx. pipiens under laboratory conditions. By using this technique, the infective females of Cx. pipiens to W. bancrofti infection could be separated into two groups Transmitters and non-transmitters. Also, the ability of transmitter females to eject L3 larvae and the level of infective larvae and the level of infective larvae burden can be determined. Transmission experiments carried out by this technique showed that extrinsic incubation period for transmission L3 larvae by Cx. pipiens is different from feeding technique, it was indicated that a high number of the non- transmitter females harboured L3 larvae in their proboscis, i.e. not every infective female is capable of ejecting L3 larvae during feeding and hence, is not able to infect man under experimental conditions. It was indicated that the transmitter female of Cx. pipiens is found to harbour significantly higher L3 larvae than the non-transmitter one but the number of transmitter females is less than that of non transmitter ones. Ejection of L3 larvae from the mosquito vectors during filaria transmission was tested under different stimulating media. Seven different media were tested. Based on the present results, precise informations about the preferred media that stimulate the ejection of L3 larvae from infected Cx. pipiens females were obtained. It was found that, sweat is the most preferred medium which could stimulate the ejection of infective filarial larvae [L3], followed by the mixture of sweat and blood while no ejection occurred in air medium. The third medium was a mixture of sugar solution and+ blood+then+ sugar+ rsolution+ and+ lastely+ water