Comparative effect of tiaprofenic acid and piroxicam alone and as adjuvants to praziquantel in schistosoma mansoni infected mice

Non-steroidal anti-inflammatory drugs [NSAIDs] [tiaprofenic acid and piroxicam] have been used alone and as adjuvants to praziquantel in treating hepatic granuloma in Schistosoma mansoni infected mice. Granuloma diameter, histamine metabolism, cyclic AMP and PGE2 levels have been measured at the end of treatment. Tiaprofenic acid effectively reduced the size of hepatic granuloma, significantly increased histamine forming capacity, CAMP level and significantly decreased plasma PGE2 level. Piroxicam had no significant effect on granuloma size, significantly decreased histamine forming capacity, CAMP and PGE2 levels. The combined therapy of either of the drugs with praziquantel did not result in an additive effect

Detection of circulating trichinella pseudospiralis antigen by enzyme linked immunosorbent assay [ELISA]

A modified double antibody sandwich ELISA was used as a sensitive technique for demonstrating the appearance of circulating antigen of T. pseudospiralis in the sera of infected mice. The sera were tested at 5 days and 2, 4, 6, 7, 8, 10 and 12 weeks PI. The antigen was first detected on the 5th day PI; its level increased to reach the 1st peak at 4 weeks, then declined at 6 weeks. A 2nd peak was observed at 8 weeks, followed by a sharp decline to give negative OD reading at 10 weeks and increased to a moderate level at 12 weeks PI

Elisa Screening for lyme disease in children with chronic arthritis

100 patients aged 6 - 15 years presented to El-Shattby University Children's Hospital with chronic/recurrent arthritis and skin lesions suggestive of erythema chronicum migrans [ECM] were investigated for presence of IgG antibodies against Borrelia burgdorferi flagellum antigen using ELISA test. Four cases yielded positive serum samples which were true positive cases as they showed negative Venereal Disease Research Laboratory [VDRL] test. Ticks collected from domestic animals from the houses of serologically positive patients were found belonging to hard tick, Rhipicephalus sanguineus. Based on clinical ground, only one case was strongly suspected to have Lyme disease due to the presence of the distinctive skin lesion [ECM], while the other 3 cases were not diagnosed till the results of serological test. This study provided serological evidence concerning the presences of Lyme disease in Egypt. Furthermore efforts to identify its prevalence. Various clinical presentations, vectors and animal hosts are warranted

Studies on T spiralis and T pseudospiralis larvae recovered from mice immunized with heterologous trichinella antigen

In this study, S3 antigen of T. spiralis and T. Pseudospiralis was given to the heterologous infection in albino mice. The recovered larvae were studied as regards their number, length, and steroscan examination. It was found that vaccination of mice with T. spiralis [S3] antigen induced reduction in T. pseudospiralis larval count by 66.43% and significant reduction in length. Using T. pseudospiralis [S3] antigen reduction of 74.77% in T. spiralis larval count was detected as well as significant reduction in length, scanning electron microscopic [SEM] examination revealed more or less similar finding in both species; loss of integrity of the cuticle, some of the larvae were attacked by granulocytes mainly eosinophils and few larvae appeared completely destructed with loss of normal architecture. A peculiar finding was noticed in case of T. pseudospiralis larvae in form of fine electron dense deposits giving the cuticular surface a mottling appearance

Scanning electron microscopic study of trichinella pseudospiralis muscle phase after modulation of host response using an immunostimulant drug

In a previous study, evident collagen deposition around T. pseudospiralis larvae was noticed after administration of Levamisole to infected mice. The present investigation was undertaken to confirm these findings. Scanning electron microscopic examination revealed multiple clear cystic formations on the surface of the diaphragm muscle. The presence of such structures was seen to be more frequent when the drug was given as early as the 6th day post infection. Membrane like structure was observed around T. pseudospiralis larvae, an interesting finding which was by far not reported before

Immunization against schistosoma mansoni by using trichinella spiralis and trichinella pseudospiralis antigens

The antigenic fraction of a cell free homogenate of each of Trichinella spiralis and T. pseudospiralis were prepared to be used for induction of immunity against S. mansoni in albino mice. The results of the study showed that immunization with either of the two antigens induced reduction in worm load ranging from 80. 48% to 87, 86% in the different groups of the study. Reduction in tissue egg count was also reported: Liver egg count was reduced from 69. 47% to 100%. Intestinal egg count reduction ranged from 73. 83% to 99. 78%. There was also reduction in egg excretion as evidenced by diminution in stool egg count. Liver granulomas were less in number and size and showed accelerated reaction together with inhibition of fluorescence. These findings were more manifest in animals given T. spiralis than T. pseudospiralis antigen