Isolation, purification and structural analysis of a polysaccharide MDG-1 from Ophiopogon japonicus / 药学学报

<p><b>AIM</b>To separate and purify the anti-myocardial ischemic polysaccharide fraction with a homogenous molecular weight from Ophiopogon japonicus, then study the chemical structure of the parts.</p><p><b>METHODS</b>Crude polysaccharides were prepared by extracting the tube root fraction of Ophiopogon japonicus with water, then precipitation with ethanol. From the crude polysaccharides, the polysaccharide of MDG-1 was separated and purified using ultrafiltration, DEAE Sepharose FF and Sephadex G-25 column chromatography. Its structure was studied by complete hydrolysis, periodate oxidation, Smith degradation, methylation analysis, 1H NMR and 13C NMR analysis etc.</p><p><b>RESULTS AND CONCLUSION</b>MDG-1 was a water-soluble beta-D-fructosan, containing a backbone composed of Fruf (2 --> 1), and a branch of Fruf (2 --> 6) Fruf (2 --> per average 2. 8 of main chain residues. Mn, Mw and Mp of MDG-1 were 3 400, 4 800 and 5 000, respectively. MDG-1 contains trace of Glc, which maybe connect to its reducing terminal. Molar ratio of Fru and Glc is approximately 35: 1.</p>

Structural features of a neutral heteropolysaccharide CPB-4 from Cynanchum paniculatum / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical features of CPB-4, a heteropolysaccharide obtained from Cynanchum paniculatum.</p><p><b>METHOD</b>Sugar composition analysis, methylation analysis, partial hydrolysis and carbon-13 nuclear magnetic resonance were used to determine the sugar composition, linkages, main chain, branch chains and branching points.</p><p><b>RESULT</b>CPB-4 is composed of L-arabinose, L-xylose, L-rhamnose and D-galactose in closely molar ratios of 0.8:0.2:0.2:1.0. Its main chain is comprised of 1, 5 linked galactose and side chains are comprised of terminal xylose, terminal arabinose, oligosaccharide of arabinose and oligosaccharide of arabinose, rhamnose and galactose. The branching points are located at C-6 and C-2 of galactose.</p><p><b>CONCLUSION</b>CPB-4 is a new heteropolysaccharide from C. paniculatum.</p>

Chemical studies on SC3, a polysaccharide from Salvia chinensis / 药学学报

<p><b>AIM</b>To study the chemical structure of SC3, an acidic polysaccharide from Salvia chinesis.</p><p><b>METHODS</b>Based on chemical (including sugar composition analysis, methylation analysis, uronic acid reduction and partial acid hydrolysis) and spectral analysis (IR, NMR, ESI-MS), the structural characterization of SC3 was investigated.</p><p><b>RESULTS</b>SC3 was composed Rha, Ara, Gal and GalA, with its mean molecular weight of 7.7 x 10(4). By means of methylation analysis, partial acid hydrolysis, NMR and ESI-MS spectrum, the linkages and sequence information of SC3 were obtained.</p><p><b>CONCLUSION</b>SC3 is an complicated acidic polysaccharide, obtained for the first time from the plant.</p>