Effect of acteoside on delaying senescence and improving immune function in SAMP8 mice / 中国药理学通报

Aim To investigate the effect of acteoside on the changes of immune function and aging process in SAMP8 mice via detecting the proportion of immune cells and the changes of immune factors. Methods Sixty male healthy SAMP8 mice and twelve SAMR1 mice were randomly divided into six groups. They were administered by gavage once a day for 90 days. Aging scoring test was conducted regularly. The immune cells and immune factors in plasma and liver were detected. Results Compared with model group, acteoside could increase CD4+lymphocytes in SAMP8 mice and effectively inhibit the increase of CD8+lymphocytes; it played a greater role in spleen than in peripheral blood. At the same time, Thl/Th2 was significantly reduced in model group, and the ratio of Thl/Th2 factor increased in low and medium dose group. Conclusions Acteoside can increase the number of helper lymphocytes, effectively inhibit the increase of killer lymphocytes, and regulate the dynamic balance of Th1 and Th2 type immune inflammatory factors in SAMP8 mice, so as to improve the immune function of the body and delay the aging process of the body.

Diagnosis of spine and spinal cord deformity in children with MRI / 中华实用儿科临床杂志

Objective To discuss the magnetic resonance imaging(MRI) characteristics of spine and spinal cord deformity in children.Methods Thirty-three children with spine and spinal cord deformity underwent MRI examination from Jan.2010 to Dec.2012.The technique was to obtain spin echo(SE) sagittal T1WI and fast spin echo(FSE) T2WI as well as axial FSE T2WI.Coronal FSE T2WI was added when necessary.Results There were 17 cases with spine deformity,accounting for 51.5％ of all patients,12 cases with both spine and spinal cord deformity,accounting for 36.4％ of all patients,4 cases with sacral canal cyst,accounting for 12.1％ of all patients.There were 4 cases of scoliosis,22 cases of scoliosis accompanying vertebral or spinal cord deformity,2 cases of atlas assimilation and basilar impression,1 case of bifid spine of the sacrum.Conclusions There are a great variety of paediatric spine and spinal cord deformities,and they always coexist.MRI can supply accurate diagnosis and useful information for clinic.

The value of gastric bare area, left adrenal gland and perirenal space involvement in predicting complications of acute pancreatitis / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate CT findings and incidence rate of gastric bare area involvement (GBAI), left adrenal gland involvement (LAGI) and perirenal space involvement (PSI) in acute pancreatitis, and to also explore the value of these appearances in predicting complications and mortality of patients.</p><p><b>METHODS</b>CT imaging data of 575 patients with AP diagnosed by clinic from October 2009 to April 2011 were analyzed retrospectively. There were 339 male and 236 female patients, aging from 16 to 93 years with a mean of (51 ± 16) years. Involvement with or without gastric bare area, left adrenal gland, perirenal space were focused, and the relationship were analyzed between these CT findings and complications and mortality of patients.</p><p><b>RESULTS</b>Among 167 patients (29.0%) with GBAI, 132 had complications and 16 died. The sensitivity and specificity of GBAI for predicting complications were 45.4% and 87.7%, respectively, and 84.2%and 72.8% for predicting mortality. In all 107 patients (18.6%) with LAGI, 81 had complications and 18 died. The sensitivity and specificity of LAGI for predicting complications were 27.8% and 90.8%, respectively, and 94.7% and 84.0% for predicting mortality. Among 335 patients (58.3%) with PSI, 201 had complications and 19 died. The sensitivity and specificity of PSI for predicting complications were 69.1% and 52.8%, respectively, and 100% and 43.2% for predicting mortality. Of all patients, 210 (36.5%) owned two or more positive CT findings among GBAI, LAGI, and PSI. One hundred ninety-eight of these patients had complications and 19 died, that predicted the sensitivity and specificity for complications were 68.0% and 95.8%, respectively, and 100% and 65.6% for mortality. The risk of complications in the patients with GBAI or LAGI was increased than normal gastric bare area or left adrenal gland 5.9 or 3.8 times respectively, and the risk of death was improved 14.3 or 94.5 times respectively. The risk of complications in those patients with two or more positive findings increased by 48.3 times. By analyzing receiver operating characteristic (ROC) curve, the combination of GBAI, LAGI, and PSI was the best way to predict the complications of AP. The area under the ROC was 0.819.</p><p><b>CONCLUSIONS</b>The CT imaging could effectively indicate the signs of GBAI, LAGI and PSI of AP. The GBAI. LAGI and PSI were related with the severity and prognosis of AP, and these findings could be clinical indicators for evaluating prognosis of AP.</p>

Comparison of coronary angiography with myocardial perfusion imaging in assessment of functionally relevant coronary artery lesion / 中华心血管病杂志

<p><b>OBJECTIVE</b>To evaluate the accuracy of dual-source CT coronary angiography (DSCTCA) for the depiction of functionally relevant coronary artery lesion(FRCAL), by using myocardial perfusion imaging (MPI) with single photon emission computed tomography (SPECT).</p><p><b>METHODS</b>DSCTCA, (99)Tc(m)-MIBI SPECT myocardial perfusion imaging (MPI) and conventional coronary angiography (CCA) were performed in 59 patients with clinical suspected CAD. Coronary artery diameter narrowing of 50% or greater at DSCTCA was defined as stenosis and was compared with MPI findings. CCA was served as a reference standard for DSCTCA.</p><p><b>RESULTS</b>(1) Agreement between DSCTCA and CCA was good (kappa = 0.93 for patient-based analysis, Kappa = 0.88 for vessel-based analysis). (2) DSCTCA revealed stenoses in 86 segments corresponding to 60 arteries in 34 patients. (3) MPI revealed 19 reversible, 21 partially reversible, and 5 fixed defects in 25 patients. (4) About 65.0% (39/60) of all the narrowed coronary arteries were determined to be FRCAL. Sensitivity, specificity, accuracy, positive predictive values and negative predictive values, respectively, of DSCTCA in the detection of all MPI defects were 92.0%, 67.6%, 78.0%, 67.6% and 92.0% on a per-patient basis and 86.7%, 89.0%, 88.6%, 65.0% and 96.6% on a per-artery basis. (5) ROC analysis showed that predictive value of DSCTCA in FRCAL was similar with those of CCA (AUCs = 0.80, 0.82).</p><p><b>CONCLUSIONS</b>DSCTCA can evaluate FRCAL indirectly. When DSCTCA results are negative, it can help ruled out patients with FRCAL. The positive DSCTCA results should combine MPI in predictor of myocardial ischemia.</p>

Clinical applications of routine subtraction and dual-energy subtraction cervical arteries computed tomographic angiography for cervical arteries imaging / 中国医学科学院学报

<p><b>OBJECTIVE</b>To compare with the clinical applications of routine-subtraction and dual-energy subtraction cervical arteries computed tomographic angiography (CTA) for cervical arteries imaging.</p><p><b>METHODS</b>Scanning was performed in 45 patients with clinically suspected cervical arteries disease with dual-source CT. The data of two different energy were collected only at one scanning. The data post processing include: conventional bone-removal digital subtraction (routine-subtraction) was performed with plain and 80 kV enhanced scanning. Volume render (VR) and maximum intensity projection (MIp) reconstruction were finished. Direct bone-removal digital subtraction (dual-energy subtraction) was performed with 80 and 140kV enhanced scanning that have different energy, and saving the data of subtraction. VR and MIp reconstruction were finished. The image quality, which was divided into four grades, was compared between these two groups. The effective radiation dose was also compared.</p><p><b>RESULTS</b>For normal vessels, no abnormality was found in 24 of 45 cases, with the common carotid artery and its branches clearly displayed with both two methods. The image quality was not significantly different between dual-energy subtraction CTA and routine subtraction CTA (P>0.05) . For stenotic vessels, 45 stenotic vessels in 21 cases were clearly displayed clearly with both two methods (P>0.05) . The effective radiation dose was decreased by 17.3 % for dual-energy subtraction CTA when compared with routine-subtraction CTA (P<0.01) .</p><p><b>CONCLUSIONS</b>Both routine-subtraction and dual-energy subtraction CTA can clearly display normal and stenotic vessels. The radiation exposure dose is relatively lower in dual-energy CTA. The dual-energy subtraction CTA has better effectiveness when used for non-cooperation patients.</p>

A new flavonol glycoside from Baeckea Frutescens L / 药学学报

To study the chemical constituents of the traditional Chinese herb Baeckea Frutescens L., a new flavonol glycoside, named 6, 8-dimethylkaempferol-3-O-alpha-L-rhamnoside (1), together with seven known compounds: quercetin (2), quercetin-3-O-alpha-L-rhamnoside (3), myricetin (4), myricetin-3-O-alpha-L-rhamnoside (5), gallic acid (6), ursolic acid (7) and 1,3-dihydroxy-2-(2'-methoxylpropionyl)-5-methoxy-6-methylbenzene (8) were isolated by using silica gel column chromatography, polyamide column chromatography and recrytallization. Their structures were identified on the basis of physicochemical properties and spectroscopic analysis. Among them, compounds 2-7 were isolated from this plant for the first time and compound 8 was first isolated from plant.