Résumé
Objective:To investigate the expression and clinical significance of B7-H3 and IL-21 in serum of patients with HBV-related primary liver cancer.Methods: Gathering 121 cases of HBV-related patients,50 cases of primary hepatic carcinoma of them were considered as hepatic carcinoma group,71 cases of benign group including 12 cases with acute hepatitis,21 cases of chronic moderate to severe hepatitis,20 cases of compensatory phase cirrhosis,18 cases of decompensated cirrhosis and 20 cases of healthy persons in the same period as normal control.The content of serum B7-H3 and IL-21 were detected by ELISA.HBV DNA quantitative results were analyzed by Quantitative Real-time PCR.Results: The levels of B7-H3 and IL-21 in patients with primary hepatic carcinoma were (207.60±57.16)ng/ml and(2 357.28±805.01)pg/ml,respectively,which were significantly higher than those of the normal control subjects(P<0.001).Comparison with the normal control subjects,the content of B7-H3 and IL-21 in serum of patients with different clinical types in the benign group increased significantly(P<0.001).B7-H3 and IL-21 were positively associated with each other in serum of patients with HBV-related primary liver cancer.The expression of sB7-H3 was not significantly correlated with the degree of HBV DNA replication.The expression of IL-21 was correlated with HBV DNA replication in patients with HBV associated hepatocellular carcinoma,but was not significantly correlated with the degree of HBV DNA replication.Conclusion: HBV-related primary hepatic carcinoma express sB7-H3 and IL-21 with high level.The continuous high expression of sB7-H3 and IL-21 in the body may be related to the development and prognosis of the patients.
Résumé
Objective: To investigate the effects of thyroid motilin (MTL) on migrating motor complex (MMC) in fasting rats. Methods: The distribution and quantitative expression of motilin in the thyroids were detected by immunohistochemistry and radioimmunoassay. We also observed the effect of thyriod motilin on the MMC by recording the gastrointestinal motility in conscious rats. Results: Tail vein injection of MTL increased MMC in antrum and duodenum in a dose-dependent manner, as manifested by increased contraction frequency and amplitude (P<0.05, P<0.01). However, the contraction frequency and amplitude were significantly decreased after injection of MTL-R antagonist GM109(P<0.05). GM109 could obviously block the promoting effect of MTL on MMC. Removing left thyroid significantly increased MMC cycle, contraction amplitude, and decreased contraction frequency (P<0.05). Injection of anti-MTL serum into the thyroids could significantly promote MMC and increase the frequency and amplitude of contraction (P<0.05). Immunohistochemistry demonstrated that motilin immunoreactive (MTL-IR) cells, together with calcitonin (CT) ,were observed in the thyroids, indicating the expression of MTL in thyroid C-cells. Radioimmunoassay analyses displayed that MTL level in the duodenal mucosa was 128.02 and 100.57 times those in the antrum and thyroid, respectively. Conclusion: MTL is expressed in the thyroid C-cell and participates in the regulation of MMC.