Résumé
Objective To establish a set of sound and scientific managing procedures and systems that conforms with the current needs and reform of the scientific research management methods .Methods Based on the current operation status of the scientific research management system in colleges of domestic universities ,this paper expounds and analyzes the theory and method of holistic quality management from the perspective of ISO9000 quality management system .Results Bring in the ho-listic quality management idea into the fields of college management ,especially cater to the science and technology management in college .Conclusions Provided a reference for bring in and comprehensive construction of ISO 9000 quality management sys-tem in scientific research management in colleges at universities .
Résumé
A droplet digital polymerase chain reaction ( ddPCR) method for quantifying E. coli O157:H7 by targeting rfbE gene was developed. The probe concentration in ddPCR was optimized and the linearity range, precision, limit of detection ( LOD) and limit of quantification ( LOQ) were also evaluated. The optimized probe concentration was 300 nmol/L. The ddPCR response was linear over the E. coli O157:H7 genome DNA concentration range from 4 to 1. 25×105 copies in 20 μL ddPCR system and the linear correlation coefficient (R2) was 0. 999. The ddPCR precision (RSD) was less than 5% over the DNA concentration range from 760 to 88400 copies/20 μL. The LOD and LOQ was 3 copies in 20 μL and 4 copies in 20 μL, respectively. Specificity test showed that the ddPCR was specific for detecting E. coli O157:H7. Both ddPCR and standard real time quantitative PCR showed the same results for 16 real samples of chicken meat, pork and beef, which indicated that ddPCR method was suitable for detection of E. coli O157:H7 in food.