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Objective To study the effect of psoralen isoflavone on the treatment of PC12 cells injured by Aβ and the mechanism on the effect of the psoralen isoflavones on the expression of related proteins. Methods The PC12 cells were divided into blank group, model group, E2 group, and psoralen isoflavones group by random number table method, In addition to the blank group the rest of each group culture medium were added 20μmol/L of Aβ25-35 modeling, The E2 group was added to the 10-3μmol/L oestrogen and psoralen isoflavones group for the intervention of 102-10-6 μmol/L.The proliferation rate of PC12 cells was detected by MTT assay, and the expression of APP, BACE1, ERβ, p-ERK and Aβ protein was detected by Western Blot. Results Compared with the model group, the proliferation of PC12 cells induced by 10-1μmol/L of psoralen isoflavone increased (101％ vs. 52％, P<0.01); The expression of p-ERK (0.751± 0.066 vs. 0.364 ± 0.015), ERβ(0.756 ± 0.105 vs. 0.337 ± 0.045) increased significantly (P<0.01); APP (0.382 ± 0.039 vs. 0.479 ± 0.015), BACE1 (0.517 ± 0.024 vs. 0.622 ± 0.029), Aβ (0.430 ± 0.032 vs. 0.581 ± 0.030) expression amount were significantly lower (P<0.05). Conclusions Psoralen isoflavones have a certain therapeutic effect on PC12 cells injured by Aβ.
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Objective To analyze the malaria epidemiological characteristics in Tengchong City from 2010 to 2015,so as to provide the evidence for adjusting and formulating measures in the elimination stage. Methods The malaria data were collected and analyzed by using Microsoft Excel 2010 in Tengchong City from 2010 to 2015. Results There were 1 408 malaria cases re?ported in Tengchong City from 2010 to 2015,including 1 091 cases of Plasmodium vivax infection,256 cases of P. falciparum infection,5 cases of P. malariae infection,1 case of P. ovale infection,1 case of mixed infections,and 54 unclassified cases. Totally 1 390 imported cases were recorded and 98.06%of them(1 363/1 390)were imported from Myanmar. Most of the pa?tients(n=908)were aged from 21 to 40 years,and the male to female ratio was 11.03∶1. The highest?risk populations were farmers and migrant workers. The most cases were observed in April and June,and at that time,most of the floating workers re?turned. Conclusion Imported malaria is severe in Tengchong City,and there is a great challenge to malaria elimination.
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Objective To study the prevalence of tick bites and relative factors of outpatients with fever from health care fa?cilities in Tengchong County. Methods From July to August,2014,the outpatients with fever in five health care facilities namely Tengchong Center for Disease Control and Prevention,Hehua Health Center,Jietou Health Center,Qushi Health Cen?ter and Xinhua Health Center were investigated by questionnaires. The factors related to tick bites were explored with the univari?ate analysis and multiple regression models. Results Totally 884 effect questionnaires were acquired. Among the 884 partici?pants,85(9.6%)had experienced tick bites. The frequency of tick bites was associated with locations,gender,age group,own?ership of cattle,working at foreign country,firewood cutting and lumbering in the forest(all P<0.05). The multiple regression revealed that the male and people raising cattle,working at foreign country,engaging in firewood cutting and lumbering in the forest had a more risk for tick bites. Conclusions Tick bites are common in the residents of Tengchong County. The risk of be?ing bitten varies in different populations. The local health departments should promote health education in the high?risk popula?tion to reduce the risk of infecting tick?borne diseases.
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Objective:To study the effect of Longman Ampelopsis grossedentata on the level of serum lipid. Methods:The exper-imental rats were given high-fat diet during the experiment. The rats were randomly divided into the model group, high dose group (37. 80 g·kg-1), medium dose group (12. 60 g·kg-1), low dose group (6. 30 g·kg-1) and lipid-lowering drug group (180 mg· kg-1 ) . All the groups were administrated continuously for 30 days, and the effect on body weight, serum TC, TG and HDL-C were measured. Results:Compared with that of the model group, the increase of body weight in the other groups was not significant ( P>0. 05), and all the rats had no obvious poisoning symptom. TC and TG were higher after the high-fat diet administration than those be-fore the experiment in the model group, and the differences were statistically significant (P<0. 05). After the 30-day treatment, the levels of TC and TG in the high-dose group were significantly decreased compared with those of the model group (P<0. 05). Conclu-sion:Longman Ampelopsis grossedentata shows assistant therapeutic effect on serum lipid.