Résumé
In this study, products of psoralen pyrolysis were detected using a solid pyrolysis apparatus and synchrotron radiation vacuum ultraviolet photoionization mass spectrum (SVUV-PIMS). The pyrolytic kinetics of psoralen was also studied by calculating its initial pyrolytic route in quantum chemistry. According to the findings with SVUV-PIMS, three pyrolytic products were observed, CO, C9H6O and C10H6O2. Theoretically, three fragment pathways were calculated for psoralen, in which the major primary decomposition route was de-CO, and the major secondary decomposition reaction was de-CO reaction of de-CO products.
Sujets)
Monoxyde de carbone , Chimie , Psoralène , Chimie , Température élevée , Cinétique , Spectrométrie de masse , Méthodes , Modèles chimiques , Structure moléculaire , Composés chimiques organiques , Chimie , Synchrotrons , Rayons ultraviolets , VideRésumé
<p><b>OBJECTIVE</b>To establish a full-quantified fingerprint method for analyzing Chinese herbal additives by HPLC.</p><p><b>METHOD</b>A HPLC in combination with PDA detector was applied with a phenomenex luna C18 (4.6 mm x 250 mm, 5 microm) column by gradient elution using acetonitrile-0.1% formic acid solution as the mobile phase. The flow rate was 1 mL x min(-1) and the detection wavelength was set at full spectrum scan.</p><p><b>RESULT</b>According to the selected chromatographic conditions, the full-quantified fingerprint of the Chinese herbal additive has good precision, reproducibility, stability and recovery.</p><p><b>CONCLUSION</b>The HPLC method developed for simultaneous determination of seven compounds is simple and valid. It can be used for quality evaluation and quality control of Chinese herbal additive and its processing products.</p>