Immediate reconstruction of tissue defects on eyelid caused by tumor excision / 中华整形外科杂志

<p><b>OBJECTIVE</b>To introduce the methods for immediate reconstruction of tissue defects on eyelid caused by tumor excision.</p><p><b>METHODS</b>Single or combined local skin flaps were used to repair tissue defects on eyelids after tumor excision.</p><p><b>RESULTS</b>Since 1994, 216 patients (224 sides) were treated. All flaps survived with primary healing. 149 patients were followed up for 3 months to 10 years with satisfactory results. 2 patients with squamous cell carcinoma recurred and reoperated with local skin flap again.</p><p><b>CONCLUSIONS</b>It is a simple, safe, flexible and reliable method to immediately repair the tissue defects with local flaps on eyelid caused by tumor excision.</p>

Local flap for reconstruction of nasal tip defect: functional and aesthetic evaluations / 中华整形外科杂志

<p><b>OBJECTIVE</b>To introduce the experience of reconstruction of nasal tip defect, which could obtain good functional and aesthetic results.</p><p><b>METHODS</b>Based on the aesthetic subunit principle, according to the size, shape, depth of nasal tip defect, and the conditions of surrounding skin, homologous local flap (include: frontonasal flap, bilobed flap, nasolabial flap, reforming rhomboid flap and forehead flap, etc.) was designed to cover the defect wound. The secondary defect of donor area was directly sutured.</p><p><b>RESULTS</b>A total of 83 patients with nasal tip defect were treated by local flap transfers. The area of the defect ranged between 0.6 x 0.6 cm and 2.5 cm x 2.5 cm. The nasal tip defect was successfully closed in all patients, and all flaps survived well without obvious complications. 76 out of these 83 patients were available for follow-up from 1 to 36 months. The repaired tissue was in good match with surrounding tissue, with nice nasal contour, and cosmetic results were satisfactory. No tumor recurrence occurred.</p><p><b>CONCLUSIONS</b>Based on the nasal aesthetic subunit principle, local flap could be used to reconstruct the nasal tip defect below 2.5 cm x 2.5 cm, and obtain good color, contour and texture in match with the surrounding skin. Cosmetic results were satisfactory.</p>

Discoidin domain receptors (DDRs) expression in pathological scar fibroblast formation / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the role of discoidin domain receptors (DDRs) in the formation of the keloid.</p><p><b>METHODS</b>The real-time quantitative PCR was used to compare the DDRs expression in the keloids and normal fibroblasts.</p><p><b>RESULTS</b>The level of DDR1 expression was significantly higher in keloid than in normal fibroblast (20.98 vs 4.2, P <0.01; 7.9 vs 4.23, P <0.05). The level of DDR1 expression in keloid was also higher significantly than that in hypertropic scar (20.98 vs 7.9, P < 0.01). However, the level of DDR2 expression was somewhat higher in keloid than in normal fibroblasts, the difference seemed not to be significantly in probability (358, 332 vs 278, P > 0.05).</p><p><b>CONCLUSIONS</b>DDRs may exert effect on keloid cell behaviours.</p>

Thread-burying in eyebrow combined with double eyelid construction in treatment of upper eyelid cutis laxa in the middle-aged and young / 第二军医大学学报

Objective: To introduce a method combining thread-burying in eyebrow and construction of double eyelid for treatment of upper eyelid cutis laxa in the middle-aged and young. Methods: We used 3-0 non-invasive thread for intradermal suture and fixed the eyebrow to the superciliary periost, then double eyelid construction was performed to remove the superfluous skin of upper eyelid in 23 patients with upper eyelid cutis laxa. Results: All the 23 cases obtained satisfactory clinical outcomes, with the upper eyelid cutis laxa. obviously improved. Conclusion: Thread-burying in eyebrow combined with double eyelid construction is a simple and effective strategy for treatment of middle-aged and young patients with upper eyelid cutis laxa.

The effects of chitosan on different-derived fibroblasts / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.</p><p><b>METHODS</b>The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.</p><p><b>RESULTS</b>This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).</p><p><b>CONCLUSION</b>The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.</p>

Expression of alpha-melanocyte-stimulating hormone in human split-thickness skin graft / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the expression of alpha-melanocyte-stimulating hormone (alpha-MSH) in human split-thickness skin autograft and the role of alpha-MSH in hyperpigmented process of the grafted skin.</p><p><b>METHODS</b>Immunohistochemical technique was used to detect the expression and distribution of alpha-MSH in the split-thickness grafted skin and normal skin separately.</p><p><b>RESULTS</b>The expression of alpha-MSH in most of the split-thickness grafted skin was much stronger than the control skin. The positive ratio of alpha-MSH expression was 61.1% in the split-thickness grafted skin, 11.1% in the normal skin of the donor area and 16.7% in the normal skin around the recipient area. The expression of alpha-MSH in the split-thickness grafted skin was significant high, compared with the normal skins (P < 0.01). The expression of alpha-MSH in the normal skin of the donor area was no statistic remarkably differences compared to the normal skin around the recipient area.</p><p><b>CONCLUSION</b>The results indicate that the expression of alpha-MSH may markedly increase in the split-thickness grafted skin and correlate with its pigmentation after the skin graft. Overexpression of alpha-MSH may play an important role in hyperpigmented process of the skin graft.</p>