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1.
Acta Pharmaceutica Sinica ; (12): 558-565, 2014.
Article Dans Chinois | WPRIM | ID: wpr-245046

Résumé

Single nucleotide polymorphisms (SNP) is an important molecular marker in traditional Chinese medicine research, and it is widely used in TCM authentication. The present study created a new genotyping method by combining restriction endonuclease digesting with melting curve analysis, which is a stable, rapid and easy doing SNP genotyping method. The new method analyzed SNP genotyping of two chloroplast SNP which was located in or out of the endonuclease recognition site, the results showed that when attaching a 14 bp GC-clamp (cggcgggagggcgg) to 5' end of the primer and selecting suited endonuclease to digest the amplification products, the melting curve of Lonicera japonica and Atractylodes macrocephala were all of double peaks and the adulterants Shan-yin-hua and A. lancea were of single peaks. The results indicated that the method had good stability and reproducibility for identifying authentic medicines from its adulterants. It is a potential SNP genotyping method and named restriction endonuclease digest - melting curve analysis.


Sujets)
Atractylodes , Classification , Génétique , DNA restriction enzymes , Métabolisme , ADN des plantes , Génétique , Contamination de médicament , Génotype , Lonicera , Classification , Génétique , Plantes médicinales , Classification , Génétique , Polymorphisme de nucléotide simple
2.
China Journal of Chinese Materia Medica ; (24): 3668-3672, 2014.
Article Dans Chinois | WPRIM | ID: wpr-311011

Résumé

To simply and rapid authenticate Lonicera japanica. Rapid allele-specific PCR primer was designed base on trnL-trnF 625 G/T Single nucleotide polymorphism and the PCR reaction systems including annealing temperature was optimized; optimized results were performed to authenticate L. japanica and its 9 adulterants. When 100 x SYBR Green I was added in the PCR product of 87 degrees C initial denatured 1 min; 87 degrees C denatured 5 s, 68 degrees C annealing 5 s, 30 cycle; L. japanica visualize strong green fluorescence under 365 nm UV lamp whereas adulterants without. The results indicate rapid allele-specific PCR could authenticate L. japanica and its adulterants rapidly and simply.


Sujets)
Allèles , Amorces ADN , Génétique , Contamination de médicament , Lonicera , Classification , Génétique , Réaction de polymérisation en chaîne , Méthodes , Contrôle de qualité
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