Progress in Drug Treatment for Renal Ischemic Reperfusion Injury / 中国药师

Renal ischemia reperfusion injury refers to the recovery failure of renal function induced by renal perfusion after ischemia,and resulting in a series of pathophysiological reactions.At present,there are no sovereign drugs for the treatment of renal ischemia reperfusion injury.Based on the pathophysiological characteristics of renal ischemia reperfusion injury and the latest research results at home and abroad,the article reviewed the research progress in the therapeutical drugs for renal ischemia reperfusion injury,including apoptotic protease inhibitors,P-selectin antagonists and antioxidants,which could provide reference for the effective intervention with the disease.

New progress inhypoglycemic mechanism and clinical application of Berberine / 中国糖尿病杂志

[Summary] Berberine is akind of alkaloids extracted from Chinese herb medicine in cludingphellod endron ,coptis and radix berberidis .In recent years ,the pharmacological effects of berberinewas investigated extensively including anti-infection ,anti-arrhythmia ,protection of ischemic myocardium ,anti-hypertension , anti-tumor ,anti-HIV .And there are increasing reports about its hypoglycemic effect ,but its mechanism remains unclear .Here we summarize the possible mechanism of hypoglycemic effect and clinical efficacy of berberine .

External application of Algoplaque can control phlebitis caused by peripheral indwelling needle invein / 中国实用护理杂志

Objective To explore the effect of prevention and treatment of external application of Algoplaque for controlling phlebitis caused by peripheral indewelling needle in vein for patients. Methods This research was divided into two parts,prevention and treatment. As for prevention research,patients were randomly divided into the experimental and the control groups,each group included 30 patients. In the experimental group,we applied directly external application of Algoplaque at the upper of needle puncture site of the vein and nearby the eye. In the control group,we applied the film directly to fix the indwelling needle. As for the treatment research, it was carried out in patients with occurred phlebitis, who were randomly divided into two groups,the experimental group included 30 cases of patients and the control group included 28 cases of patients. Observation time was one to five days. Results The incidence of phlebitis in the experimental group of prevention research was 23%, in the control group it was 90%. The incidence of phlebitis in the experimental group was significantly lower than that of the control group. The effective rate in the experimental group of treatment research was 96.7% and it was 67.9% in the control group. The difference was very significant. Conclusions External application of Algoplaque can effectively control phlebitis caused by peripheral indewelling needle in vein.

Optimization of attachment conditions for rabbit mesenchymal stem cells in cytodex 3 microcarrier culture systems / 生物医学工程学杂志

Mesenchymal stem cells are of great value in tissue engineering and genetic engineering. To study the in vitro dynamic expansion of mesenchymal stem cells by microcarrier technology, we began the research with the investigation of attachment of rabbit mesenchymal stem cells on cytodex 3 microcarriers after inoculation. The result showed a poor attachment efficiency of 16.7% +/- 1.1% under general conditions, so the attachment efficiency must be increased through the optimization of inoculation conditions. Intermittent stirring, inoculation in 50% of the final culture volume and reduction of the fetal bovine serum concentration at inoculation all led to notable increases in attachment efficiency. Ratio of rMSCs attached in aMEM cultures was 39.8% higher than that of DMEM's on the average. When all these optimal conditions were adopted, the attachment efficiency (65.5%) was significantly higher than that (26.6%) under general conditions; accordingly the final growth extents of rMSCs were 4. 50 and 2. 01, respectively. Therefore, the optimization of attachment conditions promoted the expansion of rMSCs on microcarriers.

ELECTROCHEMICAL BEHAVIOR OF OFLOXACIN AT Pt/GC ION IMPLANTATION MODIFIED ELECTRODE AND ITS APPLICATION / 药学学报

AIM　To study the electrochemical behavior of ofloxacin at Pt/GC ion implantation modified electrode. METHODS　With Pt/GC ion implantation modified electrode as working electrode, the behavior of ofloxacin was studied by voltammetry in 0.40 mol*L-1 KCl solution. RESULTS　A sensitive reductive peak of ofloxacin was obtained by linear sweep voltammetry. The peak potential was -1.35 V (vs SCE). The peak current was proportional to the concentration of ofloxacin over the range of 1.0×10-6-3.0×10-5 mol*L-1 with the detection limit of 5.0×10-7 mol*L-1. The behavior of reduction wave was studied and applied to determination of ofloxacin in tablets. CONCLUSION　The reduction process was irreversible. The element composition, atomicity form and depth of distribution at the surface of Pt/GC electrode were determined by Auger electron spectroscopy (AES), X-ray photoelectron spectroscopy (XPS) and scannig electron microscope (SEM). The catalysis behavior and reaction mechanism at Pt/GC modified electrode was also studied.