Neonatal tricho-hepato-enteric syndrome caused by SKIV2L gene mutation: a case report / 中华围产医学杂志

We here report the diagnosis and treatment of tricho-hepato-enteric syndrome in a female neonate. The 11-day-old patient, born at a gestational age of 38 weeks and with a birth weight of 1 700 g, was admitted to the Affiliated Hospital of Jining Medical University in January 2019 due to "skin stained yellow for 6 d". She presented with yellow, thin, and sparse hair that was easy to fall off, intractable diarrhea, repeated fever, and slow weight gain, further complicated by congenital heart disease. After 25-days of treatment, the child's infection was under control, but still had diarrhea. The baby girl was discharge later on request of her parents, but readmitted at the age of 3 months due to pulmonary infection. Delayed development, malnutrition, prominent forehead, wide eye distance, low nasal bridge, hepatomegaly, and intractable diarrhea were also observed. Whole exome sequencing identified a homozygous mutation of c.2344delC(p.His782fs) in SKIV2L gene in the baby, and both her parents were heterozygous carriers of the mutation at this site. She was diagnosed with SKIV2L gene mutation-induced tricho-hepato-enteric syndrome. The patient suffered from sustained diarrhea and recurrent infection and died of infection at 4 months of age after her parents' decision to withdraw treatment.

Construction and screening effective sequence of shRNA targeting human trefoil factor 3 / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#Trefoil factor 3 plays a pivot role in oncogenic transformation, growth and metastatic extension of solid tumours besides mucosal protection. We screened the best siRNA sequence targeting human TFF3 by the transient-transfection of the lentiviral mediated shRNA into thyroid carcinoma K1 cells which secrete TFF3 themselves.@*METHOD@#Four siRNA transcription template hairpin structure target potential sites in human TFF3 mRNA sequence(132,170,258 and 537 bp,seperately) were selected and synthesized,as well as one negative shRNA(shRNAC). After annealing in vitro, insert pLVX-shRNA-puro construct recombinant plasmid, then enzyme digestion and sequencing analysis. The lentiviral-shRNAs were transient-transfected into K1 cells. TFF3 mRNA and protein levels were test by real-time PCR and western blot respectively in K1 cells at 48h post transient-transfected.@*RESULT@#Genetic mutations in two sequences of shRNA1~2, so the follow-up study terminated. The TFF3 expression were obviously inhibited in K1 cells at 48 hours post transient-transfected of shRNA3 and shRNA4. TFF3 (258-276) showed the highest silencing efficiency (TFF3 mRNA reduced 60.67% and TFF3 protein reduced 56.44%, P < 0.01) when the transfection efficiency was 76.83%.@*CONCLUSION@#pLVX-shRNA-puro-TFF3 expression plamid were successfully constructed and the highest efficiency sequences were screened. All these laid a foundation for further study about the function of TFF3 gene.

Application and design of a new prone position headrest to reduce complications caused by improp-er body position after vitrectomy / 中国实用护理杂志

Objective To improve patient postoperative comfort of vitrectomy with tamponnade in the prone position, design a new prone position headrest to reduce complications caused by improper body position and observe its clinical effect. Methods According to the postoperative position of the patients, 360 cases were collected. The patients were divided into the control group and the observation group with 180 cases of each group. Observation group was treated with the new prone position headrest nursing, control group were treated with routine prone position. The comfort of patients, postoperative adverse reactions, success rate of retina reattachment were observed. Results According to simplified comfortable situation scale, physiological, psychological, social culture and environment of each individual score respectively was (2.74±0.21), (3.54±0.29) , (3.25±0.23), (3.36±0.27) points in observation group and (2.30± 0.19), (2.92±0.31), (2.93±0.26), (2.79±0.30) points in control group, and there were significant differences (t=12.368-20.845, all P<0.05). The daily posture duration in postoperative first time and 5 days was respectively (220.00±25.08), (1008.00 ± 20.32) min in observation group and (85.00±28.07), (650.00± 30.12) min in control group, and there were significant differences(t=48.117, 133.194, all P<0.01). The incidence of corneal edema, conjunctival congestion, water turbidity in observation group were lower than those in control group at 4 weeks after surgery, and there were statistically significant difference (U=6.308,8.130, 6.875, P < 0.01). The incidence of high intraocular pressure, recurrent retinal detachment rate and reduction rate in observation group were lower than those in control group at 4 weeks after surgery, and there were statistically significant difference (χ2=9.000, 10.540, 11.770, P < 0.01). Conclusions The new prone headrest can effectively improve the resection of vitreous body with tamponade patients in comfort, ensure the operation effect.

The effect of TFF3 on the proliferation and migration of papillary thyroid carcinoma K1 cell / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the effect on proliferation and invasion of human papillary thyroid carcinoma K1 cells by application of small hairpin RNA (shRNA) silencing TFF3 gene expression.@*METHOD@#Using liposome transfection method, TFF3-shRNA targeting of TFF3 gene will be transient transfected to papillary thyroid carcinama K1 cells, inducing the corresponding gene silencing. The experiment set up blank control group (Con group), negative control group (ConNC group) and interference group (TFF3-shRNA group). The TFF3 protein and mRNA expression were evaluated by RT-PCR, Real time-PCR, immunocytochemistry and Western blot in K1 cells after TFF3-shRNA transfected. CCK-8 method and Scratch test were used to detect the change of proliferation ability and invasion ability respectively.@*RESULT@#(1) The recombinant plasmid Ca # HSH018037-4-HIVmU6 carrying TFF3-shRNA transfected K1 cells successfully. (2) RT-PCR and Real time-PCR detected the expression of TFF3 mRNA, which was 0.38 ± 0.11 times as many as the blank control group (P 0.05). (3) Western blot show that after TFF3 gene silence induced TFF3 protein expression levels have decreased 59.5% (P < 0.01), The difference was statistically significant compared with the blank control group. (4) Cell scratch detects K1 cell invasion ability. The invasion ability of K1 cells in interference group (TFF3-shRNA group) reduced. The scratch width significantly decreased 57.1% than blank control group (P < 0.01). (5) CCK-8 kit detect cell proliferation ability. K1 cells grow significantly slower in the interference group (TFF3-shRNA group) than the blank control group through the analysis of the growth curve (P < 0.01). In the interference group (TFF3-shRNA group) proliferation inhibition rate of K1 cells at 6 h, 12 h, 24 h and 36 h, 48 h are 16.6%, 26.6%, 33.6%, 33.8%, 35.0% respectively. Compared with negative control group, proliferation ability of K1 cell decreased significantly.@*CONCLUSION@#Silenced TFF3 gene can cause the degradation of mRNA, reduce the protein translation , and inhibit the invasion and proliferation ability of K1 cell.

Determination of Residual Methanol in Sucralose by Gas Chromatography / 中国药房

OBJECTIVE:To establish a method for the determination of residual methanol in sucralose. METHODS:Head-space capillary GC method was adopted. The determination was performed on DB-624 capillary column by temperature program-ming(initial temperature of 35 ℃,maintaining for 5 min,then raising to 200 ℃ at a rate of 50 ℃/min,maintaining for 5 min), the detector was FID. The temperature of the injector was maintaining at 220 ℃ and that of detector at 250 ℃,carrier gas was ni-trogen gas at the flow rate of 2.0 ml/min,the split ratio was 10∶1,headspace temperature was 80 ℃ and equilibrium time was 30 min,and the volume was 1 ml. RESULTS:The linear range of methanol was 98.99-296.96 μg/ml(r=0.999 9);RSDs of preci-sion,stability and reproducibility tests were all no more than 2.16%;the average recoveries were 100.20%(RSD=0.48%,n=9). The limit of detection(LOD)of sucralose was 1.39 μg/ml. In samples from 3 manufacturers,the contents of residual methanol in one manufacturer were 3.12 μg/ml,and the other two were lower than LOD. CONCLUSIONS:This method is simple and fast, and suitable for the determination of residual methanol in sucralose.

Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-4 in human papillary thyroid carcinoma and their significances / 解剖学报

Objective To investigate the expression of fibroblast growth factor-2 (FGF-2) and fibroblast growth factor receptor-4 ( FGFR-4 ) in the papillary thyroid carcinomas ( PTC ) and clinical significance . Methods Immunohistochemistry and Western blotting for the expression of FGF-2 and FGFR-4 were performed in 89 cases of PTC and 30 cases of normal thyroid tissues ( NTT) adjacent to the tumors .Results Immunohistochemistry results showed that , FGF-2 and FGFR-4 expressions were high in thyroid carcinoma (P0.05).Analyzed by Western blotting technique ,FGF-2 and FGFR-4 expressions in thyroid carcinoma were significantly higher than that in normal tissue ,with decrease of cancer degree of tissue differentiation and significantly up regulated expression (P<0.05).Expressions of FGF-2 and FGFR-4 were in a positive linear correlation in the disease (rs=0.434,P<0.01).Conclusion The expressions of FGF-2 and FGFR-4 are correlated with papillary thyroid cancer and they participated in the process of invasion and metastasis , both of which have a positive synergistic effect .The degree of malignancy and biological behavior are meaningful and comprehensive indicators ,which provide a theoretical basis for the subsequent experimental studies of cellular and molecular biology .

Location of trefoil factor 3 in chromophil cells of rat adenohypophysis / 解剖学报

Objective To investigate the distribution and expression of trefoil factor 3(TFF3) in the eosinophilic cells and basophilic cells in the pars distalis of the rat pituitary .Methods The immunohistochemiscal staining technique was used to show the coexpression of TFF3/growth hormone (GH),TFF3/prolactin (PRL),TFF3/thyrotroph (TSH), TFF3/adrenocorticotropin (ACTH),TFF3/follicle stimulating hormone (FSH),TFF3/luteinizing hormone (LH) in two contiguous slices .Results The immunoreactive products of TFF 3 and chromophil cells were brown granules , mainly expressed in cytoplasm .ACTH positive signals were expressed in the cell membrane and mainly located in the pars distalis hypophyseos.TFF3 existed in parts of GH,PRL,TSH,ACTH,FSH and LH cells in contiguous slices, accounting for 19.4%, 22.4%, 9.2%, 6.5%, 35.7%, 8.3%, respectively , in which FSH was the most , PRL and GH were less . Conclusion TFF3 expresses in GH, PRL, TSH, ACTH, FSH, and LH cells in the pars distalis , which enriches the morphological data for the location of TFF 3 in gland cells of pars distalis hypophyseos .

Value of VEGF-C, VEGF-D and VEGFR-3 levels combined with serum TSH in diagnosis of papillary thyroid carcinoma / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate serum vascular endothelial growth factor-C (VEGF-C), VEGF-D and VEGFR-3 levels in patients with papillary thyroid carcinoma (PTC) and analyze their relation with the clinicopathological and thyroid function of the patients.</p><p><b>METHODS</b>Serum samples and the data of thyroid function were collected from 55 patients with PTC and 24 with benign thyroid tumor (BT). ELISA was used to detect VEGF-C/D and VEGFR-3 concentration in the serum samples and their relation with the thyroid function was analyzed.</p><p><b>RESULTS</b>The VEGF-C and VEGFR-3 levels were significantly higher in PTC group than in BT group (P<0.05), but VEGF-D level was comparable between them (P>0.05). In PTC patients, the elevation of serum VEGF-C and VEGFR-3 levels was associated with an advanced clinical stage (III-IV), elevated thyroid-stimulating hormone (TSH) level, an age over 45 years, and a tumor diameter exceeding 2 cm (P<0.05 or P<0.01). Patients with lymph node metastasis had significantly higher VEGF-C level but lower VEGF-3 level than those without metastasis regardless of gender. Serum VEGF-D level was higher in PTC patients with lymph node metastasis (P<0.05) and elevated TSH level (P<0.01) without association with the clinical stage, tumor diameter, age, or gender. The area under ROC curve (AUC) of serum VEGF-C, VEGFR-3 and TSH was 0.803, 0.734 and 0.707 respectively (P<0.01), and that of VEGF-D was 0.556 (P>0.05); when combined, serum VEGF-C, VEGFR-3 and TSH showed an AUC of 0.862 (P<0.01).</p><p><b>CONCLUSION</b>Detecting serum VEGF-C and VEGFR-3 levels combined with TSH may enhance the early diagnosis rate of papillary thyroid carcinoma.</p>

The expression and significance of trefoil factor 3 and SDF-1/CXCR4 biological axis in papillary thyroid carcinoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the expression of trefoil factor 3 (TFF3)with stromal cell-derived factor (SDF-1) and its receptor (CXCR4) in papillary thyroid carcinoma(PTC), and investigate the function of TFF3, SDF-1/ CXCR4 and the relationship among them during the tumor genesis,development and outcome of PTC.@*METHOD@#Detecting the expression of TFF3 and SDF-1/CXCR4 by immunohistochemical method (SP) in 92 cases of PTC and para-carcinoma tissue. Semiquantitative analysis of the results of immunohistochemistry was conducted by image analysis software.@*RESULT@#(1) TFF3 protein was expressed in the cytoplasm of cancer cells,while TFF3 was negative or weakly positive in follicular cells of para-carcinoma tissue. The positive expression rate of TFF3 was 92.39%, of which the strong positive rate of clinical stage III-IV accounted for 71.79% (42/59) and that of clinical stage I-II was 33.33% (11/33) (P < 0.01). The positive rate of TFF3 was significantly higher in the cases with lymph node metastasis than those without lymph node metastasis (100.00% vs 86.27%, P < 0.05). The AOD value of TFF3 was higher in PTC than in para-carcinoma tissue, that in cases with lymph node metastasis was higher than those without lymph node metastasis, and that in stage III-IV was higher than those in I-II (P < 0.05 or P < 0.05). (2) There was high expression of SDF-1 in the cytoplasm of malignant tissues. The para-carcinoma tissue was weakly positive or negative to SDF 1 and metastatic lymph nodes was weakly positive to SDF 1. The positive rates and AOD values of SDF-1 protein were similar to those of TFF3 in PTC,that is to say the positive rate and AOD values were higher in PTC than in para-carcinoma tissue, those in cases with lymph node metastasis were higher than those without lymph node metastasis, those in stage III-IV were higher than those in I-II, and those in patients older than 45 years old was obviously higher than those in patients under 45 years old (P < 0.05 or P < 0.01); CXCR4 was also mainly expressed in cytoplasm with few expression in nuclei, while negative or weakly positive in para-carcinoma. The positive rate and AOD values of CXCR4 in PTC were similar to SDF-1, meaning that they were higher in PTC than in para-carcinoma tissue and associated with the clinical stage, lymph node metastasis and age (P < 0.05 or P < 0.01). (3) There was positive relationship between TFF3 and SDF-1 as well as between SDF-1 and CXCR4 in PTC (r = 0.971, P < 0.01).@*CONCLUSION@#The high expression of TFF3, SDF-1 and CXCR4 in PTC are correlated with carcinogenesis and progression, and may play a significant role in evaluating the malignancy degree and progression of PTC.

Expression and Clinical Significance of TFF3 and SDF-1 Proteins in Papillary Thyroid Carcinoma / 天津医药

Objective To investigate the expression and significance of trefoil factor 3 (TFF3) and stromal cell de-rived factor-1 (SDF-1) in papillary thyroid carcinoma (PTC) and adjacent tissues, and to provide the basis for clinical diagno-sis and prognosis evaluation. Methods The expressions of TFF3 and SDF-1 proteins in PTC and adjacent tissues were de-tected by immunohistochemical SP method. The relationship between expressions of TFF3, SDF-1 and clinicopathological features was analyzed. Results (1) The positive rate and the expression level of TFF3 protein were significantly higher in PTC than those in adjacent tissues, which were higher in patients with lymph node metastasis than those in patients without lymph nodes metastasis, and were higher in PTC patients of stageⅢ/Ⅳthan those in patients of stageⅠ/Ⅱ(P＜0.05). The strong positive rate of TFF3 protein in stageⅢ/Ⅳof PTC patients was higher than that in stageⅠ/Ⅱ(P＜0.01). (2) The posi-tive rate and the expression level of SDF-1 protein were significantly higher in PTC than those in adjacent tissues, which were higher in patients with lymph node metastasis than those in patients without lymph nodes metastasis, and were higher in PTC patients of stageⅢ/Ⅳthan those in patients of stageⅠ/Ⅱ, and were significantly higher in patients over 45 years old than those of patients≤45 years old (P＜0.05). (3) There was a positive correlation between expression levels of TFF3 and SDF-1 proteins in PTC (r=0.266,P＜0.05). Conclusion The higher expressions of TFF3 and SDF-1 in PTC were closely correlated with carcinogenesis and progression, which may play a significant value in judging the malignant degree and pro-gression of PTC.

The serum TSH,TgAb,TpoAb value to the diagnosis of thyroid diseases / 重庆医学

Objective To investigate the serum levels of Thyroid stimulating hormone (TSH ) ,Thyroglobulin antibody (TgAb) and Peroxidase antibodies(TpoAb) in patients with different thyroid diseases and to explore the relationship between the 3 indexes with thyroid diseases .Methods 383 cases of thyroid diseases were divided into six groups ,simple adenoma of throid group(TA , n=30) ,nodular goiter group(NG ,n=59) ,papillary thyroid hyperplasia group(PTH ,n=36) ,nodular goiter with adenoma group (NG+ TA ,n=196) ,hashimoto′s thyroiditis(HT ,n=13) ,throid carcinoma group(TC ,n=49) .The relationship between concentra-tion of FT3 ,FT4 ,TSH ,TgAb ,TpoAb in serum and thyroid diseases incidence was analyzed .Results (1)The serum TSH concen-tration had statistically sinificance between group HT with group TA and NG ,group PTH and TA+NG group(P<0 .05) .Despite the concentration of serum TSH in TC group was in a high level near to the upper limit ,there was no statistically significance ver-sus other groups .(2)In group HT and TC ,the increased rate of TSH and positive rate of TgAb and TpoAb were significantly high-er than in group BT (P<0 .05) .(3)The double positive rate of TgAb and TpoAb ,the single positive rate of TpoAb in group HT and TC were higher than those in group BT (P<0 .05) .Conclusion There was a close correlation between serum TSH ,TgAb , TpoAb concentration and thyroid disease ,especially closely associated with HT and TC .Raised TSH and TpoAb positive have im-portant reference value in diagnosis of TC .

Detection of galectin-3 in both serum and tissue for early diagnosis of thyroid carcinoma / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the levels of galectin-3 in the serum and surgical specimens from patients with malignant and benign thyroid lesions and explore their clinical significance.</p><p><b>METHODS</b>Serum samples were collected from 159 patients with thyroid neoplasms and 16 normal subjects for detection of galectin-3 level using ELISA. The expressions of galectin-3 protein and mRNA were also detected in the surgical specimens by immunohistochemistry and in situ hybridization.</p><p><b>RESULTS</b>Galectin-3 protein and mRNA were expressed at a rate of 96.67% in 66 cases of papillary thyroid carcinoma, 83.33% in 6 cases of follicular thyroid carcinoma, 16.67% in 36 cases of papillary hyperplasia, and 11.74% in 51 cases of thyroid adenoma. The expression level of galectin-3 mRNA was slightly lower than but positively correlated with its protein expression. Patients with thyroid carcinoma had significantly higher serum concentration of galectin-3 than those with benign thyroid lesions (papillary hyperplasia and thyroid adenoma) and normal subjects (P<0.001). In patients with papillary thyroid carcinoma, galectin-3 positivity in the tumor tissue was associated with a significantly higher serum galectin-3 level in comparison with the negative cases (P<0.05).</p><p><b>CONCLUSION</b>Detection of galectin-3 in both the serum and surgical specimens can improve the diagnosis rate of thyroid carcinoma.</p>

Application of Color Doppler Ultrasonography for Superior Mesenteric Artery Diseases in the Aged / 中国康复理论与实践

@#Objective To evaluate the value of color Doppler ultrasonography for diagnosis of superior mesenteric artery diseases. Methods78 patients with abdominal pain suspected to be superior mesenteric artery diseases underwent color Doppler ultrasonography. The results were compared with other imaging methods or findings in operation. Results4 patients with superior mesenteric artery embolization coincided with the founding in operation. 18 of the 22 patients suggested with superior mesenteric artery stenoses were confirmed by CTA or DSA. ConclusionColor Doppler ultrasonography can be used in diagnosis of superior mesenteric artery diseases.

Application of Spinal Ultrasound in Children with Congenital Anomalies of Spine / 中国康复理论与实践

@#ObjectiveTo assess the value of spinal ultrasound in children with congenital anomalies of spine. MethodsUltrasound findings of 36 children with congenital anomalies of spine were analyzed and compared with the finding of corresponding MRI or operation. ResultsUltrasound finding was the same as MRI in the anatomy in 31/36 children, in addition, it also could show the pulsatile motion and blood flow signals of conus medullaris. ConclusionSpinal ultrasound can present more other informations for diagnosis for congenital anomalies of spine, especially the blood supply of conus medullaris.

Hemodynamic Change of Anterior Tibial Artery during Acupuncture at Zusanli (ST36) / 中国康复理论与实践

@#Objective To study physiological foundation of acupunctureDeqi.Methods 28 healthy volunteers who never experienced acupuncture were recruited. The right Zusanli (ST36) and a site 1.5 cm beside Zusanli (ST36) (nominated as A) were acupunctured at different time. Shallow and deep acupuncturing was inserted at Zusanli (ST36). Only deep acupuncturing was inserted at A. Hemodynamic parameters of the anterior tibial artery including the peak value in the systolic phase (Vmax), the average blood flow velocity (Vm), and the inner diameter of vessels (Dist) were observed with colored Doppler ultrasound before, at the end of acupuncture, and 3 min afterDeqi response. Results There was significant difference in those parameters between before acupuncture and 3 min after deqi response when deep acupuncturing at Zusanli (ST36) (P＜0.05), but was not significant difference when shallow acupuncturing (P＞0.05). There was significant difference in ΔVmax and ΔVm between acupuncture at Zusanli (ST36) and A points (P＜0.05). Conclusion Arterial haemodynamics variation seems to be associated with acupunctureDeqi.

Clinicopathologic and prognostic study of 404 colon cancer cases / 基础医学与临床

Objective To identify clinicopathologic characteristics and prognostic factors through a retrospective analysis of 404 colon cancer patients.Methods A total of 404 patients(209 of them were followed up with inquisition) with colon cancer were surgically treated from 1993 to 2003 and studied by univariate and multivariate analysis.Results Using univariate analysis age of patients,it was found that differentiation degree of the tumors,lymphatic invasion,abdominal and distant metastases,TNM stage and adjuvant radiotherapy were associated with outcome.(2)Multivariate analysis showed that only abdominal and distant metastases and TNM stage were associated with prognosis.Conclusion Multivariate analysis proved following list are independent prognostic factors: abdominal,distant metastases and TNM stage.

Clinical anatomy of the lingual artery / 中国耳鼻咽喉头颈外科

OBJECTIVE To provide the clinical anatomy data of the lingual artery for clinical application concerning about the lingual artery. METHODS CTA examination of the carotid artery was performed in 80 adult volunteers. The 3D reconstruction images of the carotid artery with hyoid bone were obtained by using 64-slices spiral CT. At the same time, 20 extraoral dissections of the submandibular region were performed on 10 human cadavers. The origin, pathway, and anatomic relations of the lingual artery in CTA and cadavers were studied. The distance from the origin of the lingual artery to the bifurcation of the common carotid artery and tip of the greater horn of hyoid bone were separately measured, and the distance between the segment of the greater horn of hyoid bone of lingual artery and the middle of greater horn of hyoid bone were also measured. And the contrast analysis was carried on the result. RESULTS The lingual artery was mainly come from the external carotid artery (74.4 % in the CTA; 80% in the Cadavers), the next origin was come from the facial artery (23.1 % in the CTA; 20 % in the Cadavers), and it was few to found that the lingual artery had origin in the superior thyroid artery, which was 2.5 % in CTA and none in Cadavers. The path of the lingual artery had high variation, but the position between the segment of the greater horn of hyoid bone of lingual artery and the greater horn of hyoid bone had constancy relatively. The lingual artery run forward with approximation parallel to the greater horn of hyoid bone into tongue,and which located in thesuperior to the greater horn of hyoid bone (2.32?1.29 mm) or in the inferior to the greater horn of hyoid bone (2.00?1.68 mm). The distances from the origin of the lingual artery to the bifurcation of the common carotid artery and tip of the greater horn of hyoid bone in CTA and cadavers were (12.93?7.36) mm, (10.40?5.75) mmand (14.80?6.18) mm, (8.35?5.44) mm respectively. CONCLUSION The lingual artery can be show clearly in CTA and can get the anatomy data in physiological condition with CTA.

An observation about the effects of using pillow for postoperative patients with retinal detachment by intraocular tamponade / 中国实用护理杂志

ObjectiveTo study the proper position for postoperative patients with retinal detachment by intraocular tamponade when using self-designed pillow. Methods Divided 70 postoperative patients with retinal detachment by intraocular tamponade into the experimental group and the control group randomly. The self-designed pillow was used in the experimental group, the routine nursing measures were used in the control group, and then compared the incidence rate of postoperative complication in the two groups, and evaluated the comfortable condition of the patients in the 2 groups. Results The postoperative condition in the experimental group were all better than those in the control group, P

Microorganism on Nurse Hands in General Hospital:An Investigation / 中华医院感染学杂志

OBJECTIVE To survey the varieties of microorganism on nurse hands and probe into its controlling measure in general hospital.METHODS We sampled microorganism of nurse hands during intravenous fluids infusion in the Respiratory Department,the Cardiovascular Department,the Department of Digestion,Gynecology and Obstetrics Department,the Department of Thoracic Surgery and the Department of Organ Transplantation in our hospital.All samples carried out co-cultures of fungi and bacteria.RESULTS The varieties of microorganism included not only opportunistic pathogens such as Acinetobacter,Corynebacterium,Pantoea agglomerans(Enterobacter agglomerans) former,and Escherichia coli,but also strong pathogenic microorganisms such as Staphylococcus aureus,and it is so common that finding meticillin-resistant coagulase-negative staphylococci.CONCLUSIONS Nursing staff's hands contamination is serious in the process of manipulating operation in general hospital,emphasis should be placed on prophylaxis and control for nosocomial infection.

Biological characters of islets isolated from rats and purified with Dextran T40 / 解放军医学杂志

Objective To establish a method of isolating and purifying islets from Wistar rat, and to evaluate the biological characters of the isolated and purified islets. Methods The pancreases were surgically removed from adult Wistar rats, carefully minced with scissors as small as possible prior to incubation in 0.9 mg/ml collagenase (Sigma, type Ⅴ) for 10~17min at 37℃. The digested tissue was filtered through a 60 apertures/cm~2 metal sieve and washed with 10% fetal bovine sera (FBS) in Hank’s solution. Purification was performed using Dextran discontinuous density gradient centrifugation to separate the contents of the postcollagenase pellets. Islet samples were stained with DTZ staining for identification. The viability of the purified islet was determined by acridine orange/propidium iodide (AO/PI) double staining. The in vitro function was assayed by different densities of glucose incubating islets for 45min, respectively. Insulin secretion in the supernatant was assayed by radioimmunoassay. Islets were transplanted under the kidney capsule into recipient SD rats that had been rendered diabetic by injecting streptozotocin 50mg/kg, so as to evaluate the in vivo function of islets. Results The islets were recovered between the 11% and 22% Dextran layers, and between the 11% and 1640 layers. The average number of isolated islets was 2181?14 per rat pancreas. After a discontinuous Dextran density gradient purification, about 1826?24 islets were obtained from per rat pancreas, with an average purity over 95%. Islets showed good response to glucose and high efficiency to invert hyperglycemia 7-10d after transplantation. Conclusion These results showed that the digestion methods and purification procedure which we have developed could provide islets from rats for transplantation studies. The biological characters of islets harvested by our method were very well.