Anti-inflammatory effect of astragalosides and its mechanisms of action / 中国临床药理学与治疗学

AimTo study the anti-inflammatory effect of astragalosides(AST) and its mechanisms of action. Methods The exudate volume, neutrophil count and protein content in exudate were measuredinthe carrageenan-induced air pounch model in rats. The content of PGE2 was assayed by radioimmunoassay,the activity of PLA2 by microacid titration assay, IL-8 by ELISA, and NO by nitrate reductase assay. The production of O2 in neutrophil was determined by cytochrome C assay. Results AST(40, 80 mg· kg -1) could markedly reduce the exudate volume, neutrophil count, protein content, the content of IL-8, and the production of O2.AST lowered PLA2 activity of neutrophil and accellular component in exudate, and it also decreased the contents of PGE2 and NO in exudate. Conclusion AST has an obvious anti-inflammatory effect on carrageenan-induced acute inflammation.Its mechanisms may be related to the inhibition of vascular permeability and leukocyte migration, as well as to the suppression of PLA2 activity and the reduction of IL-8, PGE2, NO and O2 production.

Inhibitory effects of alendronate on osteoclastic bone resorption in vitro / 中国药理学通报

AIM To study the effect of alendronate sodium (Alen) on osteoclastic bone resorbing lacunae. METHOD Using the method of culturing osteoclasts on slices, the techniques of photomicrography microdensitometric scan and computer image analysis. RESULTS Alen(0 5,5 or 50 ?mol?L -1 )reduced the number and the surface area of osteoctastic bone resorbing lacunae dose dependently compared with model group there was significant defference ( P