Résumé
Objective .To prove aristolochic (AA) caused vascular endothelial cells (VEC) injury via intracellular calcium overloa-ding and investigate the mechanism of calcium dobesilate antagonism. Methods Human umbilical vein endothelial cells (HUVEC) were cultured in vitro, and randomly divided into three groups: Control group, AA group, intervention group. Microscope and transmission elec-tron microscopy were used to observe changes of cell morphology and ultrastructure. ELISA method were applied to determine thrombomedu-lin (TM) in cell culture supernatant, fluorescent indicator FLuo-3/AM and intracellular calcium concentration ([Ca2+]. Results TM val-ue and average [Ca2+] i of AA group were significantly higher than that of control group (P < 0.05). Compared with the AA group, when the concentration of calcium dobesilate was 25 μM or 50 μM, TM value and average [Caz +] significantly decreased in intervention group (P < 0.05). Compared with control group, endoplasmic reticulum was pool expansion shaped, and mitochondrial cristae was absent in AA group cells. Endoplasmic reticulum and mitochondria patterns in the intervention group cells showed some improvement, compared with AA group. Conclusion AA induced VEC calcium overloading, 'I'M secretion and injury of endothelial ceils, endoplasmic reticulum and mito-chondria destruction. Dabesilate calcium could protect endoplasmic reticulum and mitochondria and reduce AA induced VEC calcium over-loading, and these could protect VEC.
Résumé
[Objective] To investigate the therapeutic effect of Radix Astragali on neonatal hypoxic-ischemic encephalopathy (NHIE) and to explore its immunoregulation.[Methods] Fifty cases of NHIE were randomized to groups A and B. Both groups were given routine treatment and group A was treated with Radix Astragali injection additionally. Symptoms and signs, scoring of neonatal behavior neurological assay (NBNA) and subtypes of peripheral blood T lymphocyte were observed before and after treatment, [Results] Time of symptom-sign disappearance was 1.4 d shorter in group A than that in group B (t = 2.06, P