Chemical Constituents of Ganoderma resinaceum / 中草药·英文版

Objective To study the chemical constituents of Ganoderma resinaceum. Methods Chemical constituents were isolated and purified by silica gel column, Sephadex LH-20, and ODS column chromatography. The structures were identified by means of physicochemical and spectral data. Results From the CHCl3 part of the fruiting bodies of this material, eight compounds were isolated. Their structures were determined to be ergosterol (1), β-sitosterol (2), ganoderic acid E (3), ganoderic acid G (4), ganoderic acid C2 (5), ganoderic acid B (6), ganoderic acid A (7), and lucidenic acid A (8). Conclusion All the compounds were obtained from this fungus for the first time.

Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum / 中国中药杂志

<p><b>OBJECTIVE</b>To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma.</p><p><b>METHOD</b>HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity.</p><p><b>RESULT</b>The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances.</p><p><b>CONCLUSION</b>This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.</p>

Effect of Xinxibao on the humoral immunologic functions of patients with rotavirus enteritis / 中国基层医药

Objective To discuss the effect of Xinxibao to rotavirus enteritis sufferers' humoral immunologic functions. Methods 260 cases rotavirus enteritis sufferers' immunoglobulin and its alexin were measured. Except conventional therapy, the treatment group plus the Xinxibao to cure, and observe two sufferer teams' clinical curative effect and the change of immunologic function. Results The acute stage sufferers' blood serum IgG, IgM, IgA, C3 were lower obviously, C4 didn't have obvious change, the activity ratio of the treatment group(81.6 % ) is obviously higher than the control group (63.5 % ). At the same time, the treatment group IgG, IgM, IgA, C3 rose obviously than before,C4 had no change. Conclusion The rotavirus enteritis sufferers' immunologic functions are disorder,Xinxibao is useful to shorten the treatment of the sufferers, and strengthen sufferers' immunologic functions in the restoration stage.

Genetic diversity of Morinda officinalis by RAPD / 中草药

Objective To investigate the genetic diversity of five populations of cultivated Morinda officinalis in Guangdong Province using random amplified polymorphic DNA(RAPD).Methods Sixty-four individuals in five populations of cultivated M.officinalis were analyzed by RAPD markers to determine the genetic variations among the populations.The data of genetic diversity were analyzed with Popgen32 software.Results The levels of genetic variation and patterns of population structure in M.officinalis were investigated using RAPD markers.Of the 100 primers screened,15 primers produced highly reproducible RAPD bands,using these primers,224 discernible DNA fragments were generated with 112(50.00%) polymorphic fragments,indicating considerable genetic variation at the species level.In contrast,there were relatively high levels of polymorphism at the population level with the percentage of polymorphic bands ranging from 37.05% to 53.13%,and the mean percentage of polymorphic loic(P=45.86%).Genetic variation among populations was detected based on Nei's genetic diversity analysis(0.175 6),Shannon's diversity index(0.287 6) for every population with Shannon's index 0.103 3—0.236 2 and Nei's indexes 0.074 5—0.154 0.Conclusion There is a little genetic differentiation among populations of cultivated M.officinalis and result in different cultivated types,the genetic diversity with M.officinalis(POP2) is higher than that of other populations.It may be the main reason of the difference of cultivated M.officinalis quality.