Résumé
This paper developed an experimental animal cabin with ATM/LP and low oxygen and high carbon dioxide. It is based on the unitized design of modern transmission technology, industrial measurement modules and configuration software. The cabin successfully simulated an enclosed environment of low oxygen and high carbon dioxide at constant pressure, automatically measured and controlled the concentrations of oxygen and carbon dioxide as well as temperature and humidity in the cabin for the first time. The system provided a technology platform for the equipment supporting clinical COPD study. The result is accurate, stable and reliable and can realize automatic measurement and control of cross. The clinical applications show that the cabin with accurate measurement and control is practical and reliable.
Sujets)
Animaux , Alternatives à l'utilisation d'animaux , Techniques de biocapteur , Dioxyde de carbone , Métabolisme , Environnement contrôlé , Conception d'appareillage , Hypoxie , Oxygène , Syndromes d'apnées du sommeilRésumé
<p><b>OBJECTIVE</b>To explore the feasibility of the animal experimental cabin to copy the animal models with ATM/LP and low oxygen and high carbon dioxide environment.</p><p><b>METHODS</b>60 SPF-class male SD rats were divided into two groups, 20 for normobaric hypoxia control experiments and the other 40 for hypobaric hypoxia control experiments. For each group, examine the pulmonary arterial pressure and carotid arterial pressure indicators of rats by using the multi-detector measurement of physiological, and observe the pulmonary vascular changes in the structure.</p><p><b>RESULTS</b>The ATM/LP and low oxygen and high carbon dioxide environment can promote the formation of pulmonary hypertension and accelerate changes in pulmonary vascular remodeling, promoting right ventricular hypertrophy.</p><p><b>CONCLUSION</b>Clinical applications showed that the animal experimental cabin was observing and controlling accurately, safe, reliable, and the results was reproducible. It can successfully copy the regular low-pressure hypoxia and hypercapnia environment pulmonary hypertension model, in order to study the physiological mechanism of a variety of circulation and respiratory diseases caused by oxygen-poor and provide an experimental technology platform.</p>