Résumé
Objective To observe the effects of intra-articular injection of sinomenine on the expressions of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) in articular cartilage and synovium of rabbit knee osteoarthritis models; To discuss its mechanism of action. Methods Totally 40 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by using intra-articular injection of papain enzyme and were treated with saline, sodium hyaluronate and sinomenine respectively for 30 d, except for the control group. After treatment, method of immunohistochemistry was used to detect the expressions of VEGF and NGF in rabbit articular cartilage and synovium. Results Compared with the control group, the expressions of VEGF and NGF in articular cartilage and synovium of model group significantly increased (P<0.01); compared with the model group, the expressions of VEGF and NGF in articular cartilage and synovium of sinomenine group significantly decreased (P<0.05, P<0.01); the expressions of VEGF and NGF in articular cartilage and synovium in sinomenine group were lower than hyaluronate group (P<0.05, P<0.01). Conclusion Intra-articular injection of sinomenine can down-regulate the expressions of VEGF and NGF in articular cartilage and synovium of rabbit knee osteoarthritis.
Résumé
Objective To observe the effects of intra-articular injection of sinomenine on morphology of synovium and cartilage as well as contents of MMP-13 and cartilage oligomeric matrix protein(COMP) in serum and synovial fluid of rabbit knee osteoarthritis model.Methods A total of 38 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by intra-articular injection of papain enzyme, and were treated with saline, sodium hyaluronate and sinomenine respectively except for control group. 5 weeks after treatment, all rabbits were sacrificed for HE staining and histological grading on cartilago articularis and synovium, and ELISA method was used to detect the contents of MMP-13 and COMP in rabbit serum and synovial fluid.Results Mankin's scores of articular cartilage and histological scores of synovium in model group were significantly higher than those of control group (P<0.01), and were markedly lower in sinomenine group than those of model group (P<0.01); the contents of MMP-13 and COMP in serum and synovial fluid of model group were significantly raised compared with control group (P<0.01), and were reduced obviously in sinomenine group compared with model group (P<0.05,P<0.01).Conclusion Intra-articular injection of sinomenine can reduce the levels of MMP-13 and COMP in serum and synovial fluid of rabbit knee osteoarthritis model, and improve synovial inflammation, as well as delay the degradation of articular cartilage.