Bactericidal Effect of Disinfectant Tego-51(R) / 병원감염관리

BACKGROUND: Disinfection is essential for the prevention of hospital infoction. Tego-51, one of the amphoteric surfactants based on the dodecyl-di( aminoethyl)-glycine, has been considered as an effctive disinfectant having a broad specturn of antimicrobial activity. We evaluated the disinfective activity of Tego-51 against several clinical isolates of bacteria and yeasts including Helicobacter pyiori. METHODS: Twenty three strains of vacteria including H. pylori, and a strain of yeast were exposed to the various concentrations (0.05%, 0.01%, 0.005%) of Tego-51 for the various periods (0.5, 1, 2, 4, 8, 16min). After the exposure to Tego-51 disinfectant, 0.01 mL of mixture of microorfanisms and Tego-51 was inoculated into brain-heart infusion broth, into Sabouraud dextrose agar. or Wilkins-Chalgren agar with 10% sheep blood, and incubated at 37 degrees C for 48 hours or in the Campy Pouch microaerophilic system. RESULTS: Most strains were killed within 30 seconds after an exposure to 0.01% of Tego-51, but Proteus mirabilis was eradicated after two minutes of exposure. At the concentration of 0.005 % concentration. P. mirabilis and Bacillus subtilis were killed after eight minutes od exposure. H. pylori was killed with 0.005% Tego-51within 30 seconds. Conslusions: This study showed that Tego-51disinfectant was effective for the disinfection of commonly isolated bacteria and yeast from hospital. It may be recommended that Tego-51 should be used at concentration greater than 0.1% for the effective disinfection of skin, instruments and hospital floors.

Report of Intravenous Fluid Contamination Originated from Rubber Caps: A Study of Contamination Routes of Intravenous Fluid / 병원감염관리

BACKGROUND: Nosocomial septicemia associated with contamination of infusate occurs infrequently. Three patients in a university hospital developed fever that was suspected to be infusion-related. These patients were receiving Hartman's solutions that were found to be contaminated by Klebsiella oxytoca, Pseudomonas species, and Citrobacter species. We evaluated the contamination routes of infusates. METHODS: Samples for culture were collected from used intravenous fluids and unused fluids, and the top of rubber caps were swapped. These were cultured in trypticase soy broth and blood agar plate. RESULTS: Cultures of used intravenous fluids showed that five of 33 fluids were contaminated by bacteria, and cultures of unused fluids yielded no microorganism. We suspected that contamination of the fluids developed during insertion of administration set, especially from top of the caps. Cultures of the caps disclosed that 26 of 40 caps were contaminated, and contamination rate was higher when caps had been moistured with water. After disinfection with 70% alcohol, culture positive rate of the rubber caps was reduced to 15.0% (6/40). Of the fluids which had standed for seven hours with administration set inserted, four were culture-positive, and two of them showed same organism obtained from their caps. CONCLUSIONS: The results of this study suggest that the rubber cap can be the source of contamination of IV fluids.

Report of Intravenous Fluid Contamination Originated from Rubber Caps: A Study of Contamination Routes of Intravenous Fluid / 병원감염관리

BACKGROUND: Nosocomial septicemia associated with contamination of infusate occurs infrequently. Three patients in a university hospital developed fever that was suspected to be infusion-related. These patients were receiving Hartman's solutions that were found to be contaminated by Klebsiella oxytoca, Pseudomonas species, and Citrobacter species. We evaluated the contamination routes of infusates. METHODS: Samples for culture were collected from used intravenous fluids and unused fluids, and the top of rubber caps were swapped. These were cultured in trypticase soy broth and blood agar plate. RESULTS: Cultures of used intravenous fluids showed that five of 33 fluids were contaminated by bacteria, and cultures of unused fluids yielded no microorganism. We suspected that contamination of the fluids developed during insertion of administration set, especially from top of the caps. Cultures of the caps disclosed that 26 of 40 caps were contaminated, and contamination rate was higher when caps had been moistured with water. After disinfection with 70% alcohol, culture positive rate of the rubber caps was reduced to 15.0% (6/40). Of the fluids which had standed for seven hours with administration set inserted, four were culture-positive, and two of them showed same organism obtained from their caps. CONCLUSIONS: The results of this study suggest that the rubber cap can be the source of contamination of IV fluids.