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Journal of Forensic Medicine ; (6): 262-267, 2008.
Article Dans Chinois | WPRIM | ID: wpr-983391

Résumé

OBJECTIVE@#To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.@*METHODS@#Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.@*RESULTS@#More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.@*CONCLUSION@#The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.


Sujets)
Analyse de polymorphisme de longueur de fragments amplifiés/méthodes , ADN des plantes/génétique , Colorants fluorescents , Génétique légale , Papaver/génétique , Polymorphisme génétique
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