Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtrer
1.
Chinese Journal of Biotechnology ; (12): 874-880, 2008.
Article de Chinois | WPRIM | ID: wpr-342823

RÉSUMÉ

We produced beta1 gene which is about 2400 bp by reverse transcription polymerase chain reaction (RT-PCR) from bovine trachea, reclaimed and purified, then cloned the amplified fragment to pGEM-T easy vector, confirmed by sequencing. The immune-dominant epitope of beta1 gene was chosen by computer analysis and then syncretized ligand-binding domain from 346 bp to 843 bp of ecytoplasm with six histidine, expressed LBD protein massly in E. coli BL21 (DE3), and identified by SDS-PAGE. The fusion protein was purified with Ni-NTA affinity chromatography and immunized New Zealand rabbits preparing of its polyclonal antibody, the specific antibody titer was above 1:12,800 detected by indirect ELISA, the result of Western blot showed that this antibody could be recognized by LBD fusion protein.


Sujet(s)
Animaux , Bovins , Lapins , Anticorps monoclonaux , Escherichia coli , Génétique , Métabolisme , Virus de la fièvre aphteuse , Physiologie , Intégrine alpha1 bêta1 , Génétique , Allergie et immunologie , Ligands , Liaison aux protéines , Motifs et domaines d'intéraction protéique , Récepteurs viraux , Génétique , Métabolisme , Protéines de fusion recombinantes , Génétique , Allergie et immunologie
SÉLECTION CITATIONS
DÉTAIL DE RECHERCHE