Résumé
The lipid content of Halocnemurn strobilaceum grown in Egypt was studied. The unsaponifiable fraction was identified by GLC. A series of hydrocarbons ranging from C[17]-C[31] in addition to campasterol, stigmasterol, beta-sitosterol and the triterpenoid cc-amyrin were identified. The GLC analysis of the fatty alcohol fraction revealed the presence of 7 fatty alcohols, also the analysis of the fatty acid fraction revealed the presence of 10 fatty acids in which palmitic acid was the main acid [39.26%]. The flavonoidal constituents isolated from the chloroform and the ethyl acetate fractions of the aqueous alcoholic extract of Halocnemurn strobilaceum were identified as Chrysoeriol, Luteolin 7-O-galactoside, Quercetin 7-O-rhamnoside, Luteolin, and a coumarinic compound, Scopoletin was also identified. Their identity was proved by m.p., TLC, PC, UV, 'H-NMR and MS analysis. GLC analysis of the volatile oil of the plant revealed the identification of 23 compounds which accounted for 63% of the oil. The oil is characterized by a high content of hydrocarbons [55.9%], while the oxygenated hydrocarbons were represented by [33.8%] of the identified components. Also, the sesquiterpene hydrocarbons and oxygenated sesquiterpene compounds were found to be 2.6% and 8.2% respectively. The radical scavenging effects of the extracts and isolated compounds on DPPH free radical were studied. The ethyl acetate extract had a strong antioxidant activity, also the isolated flavonoidal compounds showed high antioxidant activity as compared to Trolox [standard antioxidant compound]. The different extracts and isolated compounds of the plant exhibited no cytotoxic activity against Ehrlich-ascitis carcinoma cell line at the tested concentrations, except the volatile oil which showed moderate activity
Résumé
The flavonoidal constituents isolated from the chloroform and ethyl acetate fractions of aqueous alcoholic extract of Asteropterus leyseroides [Desf] were identified as quercetin, Jaceidin, jacein and a phenolic acid, caffeic acid using PC, TLC, uv,[1-]H-NMR and MS analysis. Also, the lipid content was studied. GLC analysis of the unsaponifiable fraction revealed the presence of a series of hydrocarbons ranging from C[I2]-C[29] in addition to cholesterol, stigmasterol and alpha-amyrin, as well as fatty alcohol fraction ranging from C[24]-C[39] were identified. The fatty acid fraction revealed the presence of 10 fatty acids in which linoliec acid [35.99%] represented the major constituent. The crude protein of the plant was found to be [12.71%]. The analysis of the amino acids using the amino acid analyzer revealed the presence of 15 amino acids. The mucilage hydrolysate of the aerial parts of Asteropterus leyseroides was found to contain glucouronic acid, xylose, rhamnose and glucose using PC. The radical scavenging effects of the tested extracts and isolated compounds on 1, 1 Diphenyl-2-picrylhydrazyl [DPPH] free radical were observed. The ethyl acetate fraction and the isolated flavonoidal compounds showed high antioxidant activity. The different extracts and isolated compounds of the plant exhibited no cytotoxic activity against Ehrlich-ascitis carcinoma cell line at the tested concentrations