Résumé
Objective: To assess the In vivo antioxidFant and hepatoprotective activity of methanolic extract of Daucus carota (D. carota) seeds in experimental animals. Methods: Methanolic extracts of D. carota seeds is used for hepatoprotection assessment. Oxidative stress were induced in rats by thioacetamide 100 mg/kg s.c, in four groups of rats (two test, standard and toxic control). Two test groups received D. carota seeds extract (DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin (25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle. On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) were estimated in blood serum and antioxidant enzyme like superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and lipid peroxidation (LPO) were estimated in liver homogenate. Results: A significant decrease in SGPT, SGOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group (P < 0.001) and in case of antioxidant enzyme a significant (P < 0.001) increase in SOD, CAT, GRD, GPX and GST was observed in all drug treated groups as compared with thioacetamide group. But in case of LPO a significant (P< 0.001) reduction was observed as compared to toxic control group. Conclusions: DCSE has contributed to the reduction of oxidative stress and the protection of liver in experimental rats.
Résumé
The field of probiotics is an evolving field. Probiotics were first introduced to the world as a functional ingredient in dairy products, breakfast cereals and snacks. Unknown to many, probiotics offer health benefits that go beyond the scope of supporting digestive health. Research continues to emerge, supporting the use of different probiotic strains in a variety of conditions. The research now suggests that these“healthy”bacteria have beneficial effects on gastrointestinal dysfunctions, including diarrhea, as well as the immune system and conditions such as allergy, in children, adults, and in the oral cavity. Research on the horizon will develop a better understanding of the concepts of how colonizing microbes and probiotics can influence human health.