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1.
Article Dans Portugais | LILACS | ID: lil-560255

Résumé

O objetivo foi verificar as alterações no estrato córneo em modelos alternativos de membrana após a aplicação de ativos hidratantes envolvendo métodos biofísicos. O modelo de biomembrana utilizado foi a muda de pele de Crotalus durissus e os ativos hidratantes foram: uréia, silício orgânico, extrato vegetal de Imperata cylindrica, reação de xilitol e glicose e componentes de NMF. Os resultados da avaliação das alterações do modelo por meio de Espectroscopia Raman com Transformada de Fourier sugerem que os ativos hidratantes confirmam segurança necessária, pois não alteraram de forma acentuada a estrutura do estrato córneo. Utilizando-se Calorimetria Exploratória Diferencial pode-se indicar que a solução de silício orgânico e o gel hidrofílico com uréia apresentaram melhor poder hidratante.


The objective of this research was to use biophysical techniques to investigate the alterations induced in a biomembrane model of the stratum corneum by the application of moisturizers. The biomembrane was obtained from the skin shed by the rattlesnake Crotalus durissus and the active moisturizing compounds were: urea, dimethylsilanol hyaluronate, Imperata cylindrical plant extract, carbohydrates and natural moisturizing factors (NMF components). Results from FT-Raman spectroscopy suggested that the moisturizers were safe, since they did not promote modifications in the structure of the stratum corneum. Differential scanning calorimetry results indicated that the solution containing the organic silicon compound and the gel with urea showed the best hydrating effects on the stratum corneum.


Sujets)
Humains , Analyse spectrale Raman/instrumentation , Crotalus cascavella/usage thérapeutique , Agents hygroscopiques
2.
Asian Pac J Allergy Immunol ; 2001 Dec; 19(4): 283-6
Article Dans Anglais | IMSEAR | ID: sea-37170

Résumé

Cases of food-dependent exercise-induced anaphylaxis (FEA) caused by buckwheat have been rare. Clinical, laboratory, and autopsy findings are present on an 8-year old girl with FEA caused by Japanese buckwheat. The patient consumed buckwheat noodles called "zaru soba" and immediately thereafter swam vigorously. Approximately 30 minutes later, she complained of abdominal pain, vomiting, coughing, and chest discomfort. Another ten minutes later her consciousness level deteriorated and she experienced cardiorespiratory arrest. The heart beat was restored and she was admitted to the hospital. She never regained consciousness and expired after another arrest 13 days later. Her IgE level was high (2,840 IU/ml) and the IgE-radioallergosorbent test (RAST) score was 2 for soybeans, 3 for buckwheat, 2 for rice, and 3 for wheat. An exaggerated hematemesis that occurred immediately after hospital admission indicated an inflammatory condition of the digestive tract that was caused by buckwheat. Marked ulceration accompanied with hemorrhage and necrosis was noted at the ileum. Extensive hemorrhage involving the endotracheal pulmonary field and lymphocyte infiltration of the alveolar space likely appeared after the inflammation. The analysis of buckwheat-specific IgE antibody by immunoblotting showed 7 bands that reacted with the IgE of the patient's serum, 4 bands: 16, 20, 24, and 58 kDa, were specific to the patient as compared to subjects not allergic to buckwheat. A first case of fatal FEA by buckwheat is reported with reference to specific IgE.


Sujets)
Anaphylaxie/étiologie , Enfant , Exercice physique , Fagopyrum/effets indésirables , Issue fatale , Femelle , Hypersensibilité alimentaire/complications , Humains , Immunoglobuline E/sang , Natation
3.
Asian Pac J Allergy Immunol ; 2001 Jun; 19(2): 85-91
Article Dans Anglais | IMSEAR | ID: sea-36695

Résumé

Kawasaki disease (KD) is a childhood-onset vascular disease. We assessed the concentrations of macrophage-colony stimulating factor (M-CSF) and those of lipids in sera from patients with KD. The M-CSF concentration in patients with acute-phase KD was 2,914+/-159 U/ml, significantly higher than that in control subjects with Infectious diseases (1,241+/-96 U/ml). The elevated levels of this cytokine in the acute phase fell to 1,319+/-138 U/ml in the convalescent phase. Total and high-density lipoprotein cholesterol concentrations in acute phase KD (113.8+/-8.4 and 21.5+/-2.3 mg/dl, respectively) were lower than in the infectious disease controls (195.8+/-7.0 and 62.5+/-1.8 mg/dl). The elevation of M-CSF correlated with the decrease of total and high-density lipoprotein cholesterol. Overproduction of macrophage-colony stimulating factor activates macrophages and monocytes and may disturb the lipid metabolism. Both effects could contribute to vasculitis in KD.


Sujets)
Protection de l'enfance , Enfant d'âge préscolaire , Cholestérol/sang , Cholestérol HDL/sang , Femelle , Humains , Immunoglobulines par voie veineuse/usage thérapeutique , Nourrisson , Protection infantile , Japon/épidémiologie , Numération des leucocytes , Facteur de stimulation des colonies de macrophages/sang , Mâle , Monocytes/cytologie , Maladie de Kawasaki/sang , Statistiques comme sujet
4.
Asian Pac J Allergy Immunol ; 1996 Dec; 14(2): 69-79
Article Dans Anglais | IMSEAR | ID: sea-36949

Résumé

Allergen activates T lymphocytes responsive to interleukin 2 (IL-2) in allergic patients but not in normal individuals. This response was suppressed by anti-allergic agent, Ketotifen (4-(1-methyl-4-piperidylidene)-4H-benzo [4, 5] cyclohepta [1, 2-b] thiophen-10 (9H)-one hydrogen (fumarate). Prolonged culture of antigen-presenting adherent cells impaired the ability to present Dermatophagoides farinae (Df) antigen to T cells, whereas stimulation of adherent cells with recombinant interferon-gamma (IFN-gamma) restored the antigen-presenting capability. The maintained antigen presenting ability of adherent cells treated with IFN-gamma was also suppressed by Ketotifen. Fluorescence activated cell sorter (FACS) analysis disclosed that Ketotifen selectively reduced the expression of HLA-DQ antigen, crucial restriction elements in Df antigen-related responses, on macrophages but not on B cells, even in the presence of IFN-gamma. Collectively, Ketotifen prevented macrophages from inducing allergen-activated T lymphocytes' responsiveness to IL-2 at least in part by decreasing the expression of HLA-DQ antigen.


Sujets)
Adolescent , Adulte , Antiallergiques/pharmacologie , Présentation d'antigène/effets des médicaments et des substances chimiques , Asthme/immunologie , Adhérence cellulaire/effets des médicaments et des substances chimiques , Cellules cultivées , Enfant , Enfant d'âge préscolaire , Relation dose-effet des médicaments , Cytométrie en flux , Antigènes HLA-DQ/métabolisme , Humains , Interféron gamma/métabolisme , Interleukine-2/métabolisme , Interleukine-4/métabolisme , Kétotifène/pharmacologie , Macrophages/effets des médicaments et des substances chimiques , Lymphocytes T/effets des médicaments et des substances chimiques
5.
Braz. j. med. biol. res ; 27(9): 2197-206, Sept. 1994. ilus
Article Dans Anglais | LILACS | ID: lil-144473

Résumé

1. The submandibular salivary gland of rats was observed by high-resolution scanning electron microscopy employing the aldehyde-osmium-DMSO-osmium method. 2. The intracellular membranous components and sponge-like structures of basement membrane containing the fine collagen fibrils of acinar cells were clearly identified in three dimensional images. The granular endoplasmic reticulum and Golgi apparatus showed the luminal surface. The mitochondria were small, ranging in diameter from 0.3 to 0.5 µm, and revealed their cristae. The secretory granules ranged in diameter from 0.3 to 1.4 µm. ribosome granules were attached to the surfaces of cisterns, and measured 20 to 25 nm in diameter. 3. The contact areas between the acinar cells revealed numerous cytoplasmic protrusions. In the striated duct cells, the mitochondria were arranged vertically and surrounded by nasal infoldings of the plasma membranes. At high magnification, the mitochondrial cristae were visualized in their three-dimensional characteristics


Sujets)
Rats , Animaux , Glande submandibulaire/ultrastructure , Matrice extracellulaire/ultrastructure , Appareil de Golgi/ultrastructure , Granulations cytoplasmiques/ultrastructure , Microscopie électronique à balayage/méthodes , Mitochondries/ultrastructure , Rat Sprague-Dawley , Réticulum endoplasmique/ultrastructure
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