Expression of transient receptor potential vanilloid 3 ion channel protein in human odontoblasts / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the expression of transient receptor potential vanilloid 3 (TRPV3) ion channel protein in human odontoblasts (OD).</p><p><b>METHODS</b>Twenty intact and healthy third molars extracted for orthodontic purpose were included. The quality of dental tissue sections was determined through HE staining, and the OD layer was further determined by dentin sialophosphoproteins (DSPP) antibody staining, and finally the expression of TRPV3 ion channel protein in human dental pulp tissue was examined by TRPV3 ion channel protein-specific antibody. The expression of TRPV3 channel proteins in human OD at different part of dental pulp was compared using Image Pro Plus (IPP) and SPSS software.</p><p><b>RESULTS</b>TRPV3 channel protein expressed on the cell body of OD in the coronal and root pulp, and the expression in the coronal pulp was significantly higher than that in the root pulp. The TRPV3 protein also expressed at the odontoblastic process, with the higher expression in the crown (IA = 2516 ± 162) than in the root (IA = 2224 ± 150) and external root (IA = 2121 ± 92) (P < 0.05), but the expression between the lateral root area and external root area was not significantly different (P > 0.05).</p><p><b>CONCLUSIONS</b>Human odonoblasts expressed TRPV3 ion channel protein and the expression level was different at different part of dental pulp OD.</p>

Effect of Galla chinensis on the remineralization of two bovine root lesions morphous in vitro / 国际口腔科学杂志·英文版

The present study aims to evaluate the effect of Galla chinensis compounds on the remineralization of two artificial root lesions morphous in vitro. Sixty bovine dentine blocks were divided into two groups and individually treated with two levels of demineralization solutions to form erosive and subsurface artificial carious lesions in vitro. Each group was then divided into three subgroups, each of which were treated with a remineralization solution (positive control), deionized water (negative control), or 4 000 mg⋅L(-1) aqueous solutions of Galla chinensis extract. The dentine blocks were then subjected to a pH-cycling regime for 7 days. During the first 4 days, the daily cycle included 21-h deal and 3-h demineralization applications. The dentine blocks were dealt with the entire day during the remaining 3 days. Two specimens from each of the treatment groups were selected and observed under a polarized light microscope. Data collected using a laser scanning confocal microscope were computerized and analyzed. Galla chinensis extract clearly enhanced the remineralization of both erosive lesion and subsurface lesion patterns in the specimens (P<0.05). The level of remineralization of the erosive lesion by Galla chinensis extract was lower than that of the subsurface lesion (P<0.05). In addition, the remineralization of the subsurface lesion by Galla chinensis extract was higher than that of the remineralization solution (P<0.05). No significant difference between the remineralization of erosive lesions by Galla chinensis extract and the remineralization solution was observed (P>0.05). So Galla chinensis extract has the potential to improve the remineralization of artificial root lesions under dynamic pH-cyclic conditions, indicating its potential use as a natural remineralization medicine.

Cross-sectional study of dentine hypersensitivity in smaller cities and rural area in Sichuan province / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the clinical characteristics and risk factors of dentine hypersensitivity in smaller cities and rural area in Sichuan province.</p><p><b>METHODS</b>The examinee aged 20 - 69 years old were interviewed and divided into five age groups (20 - 29, 30 - 39, 40 - 49, 50 - 59 and 60 - 69). The random sampling methods were performed in this study. A total of eight spots were survied, including 4 communities and 4 spots in rural area of Sichuan province. The information about the examinee's age, gender, occupation, education level, tooth brushing methods, the frequencies of eating fresh fruits and fruit juices and so on, were asked and recorded. All subjects were further diagnosed by a blast of air from a triple syringe connected to an air compressor at a pressure of 4 atm under room temperature of about 19 - 24°C.</p><p><b>RESULTS</b>The premolars were the most commonly affected, followed by the first molar. The exposed root surface was the most commonly affected position [63.87% (663/1038)]. The first premolar had the greatest number of teeth with dentine hypersensitivity [29.96% (311/1038)]. Different tooth had different sensitive position. Female, too much time of using a tooth brush, and hydrochloric acid in gastric juice were risk factors for dentine hypersensitivity (OR value = 2.175, 1.157, 1.760).</p><p><b>CONCLUSIONS</b>Dentine hypersensitivity is influenced by multiple factors. Prevention and treatment need be performed by improving general oral health and periodontal conditions.</p>

Epidemiological survey of dentine hypersensitivity of 630 adults in rural of Sichuan province / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the prevalence and potential risk factors of dentine hypersensitivity of adults in rural of Sichuan province.</p><p><b>METHODS</b>All representative samples, including 630 adults living in rural of Sichuan Province, were selected by multi-stage, stratified and random sampling. The dentine hypersensitivity of all 630 cases was surveyed with questionnaire and oral clinical examination. SPSS 18.0 software was used for statistical analysis.</p><p><b>RESULTS</b>27.9% of all subjects were suffered from dentine hypersensitivity, sour was the most common stimulus of dentine hypersensitivity. The first premolar was the most common tooth with dentine hypersensitive, which occupied 27.4% of the total affected teeth. Female, acid regurgitation symptom, low frequency of toothbrush replacement (over 3 months), high tooth-brushing force and frequency of fresh fruits consumption (over 2 times per week) probably were high risk factors of dentine hypersensitivity.</p><p><b>CONCLUSION</b>The prevalence of dentine hypersensitivity occurs in rural of Sichuan province is high, thus for future the publicity and education on dentine hypersensitivity preventive should be strengthened.</p>

Epidemiological survey of dentine hypersensitivity of young people in Chengdu City / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the prevalence and risk factors of dentine hypersensitivity of young people in Chengdu city.</p><p><b>METHODS</b>The dentine hypersensitivity of 741 cases aged 18-35 living in Chengdu city were surveyed with questionnaire and oral examination by random collection during June to July in 2008. The relative risk factors to dentine hypersensitivity were analyzed.</p><p><b>RESULTS</b>128 cases (17.27%) were diagnosed as dentine hypersensitivity. The prevalence of dentine hypersensitivity was higher among female than male (P<0.01). Cold was the most common stimulus for dentine hypersensitivity (62.80%). The most common affected tooth was the right maxillary first premolar (15.51%). Acid regurgitation, carbonated beverage, hard toothbrush type and heavy toothbrushing force were risk factors to dentine hypersensitivity.</p><p><b>CONCLUSION</b>The prevalence of dentine hypersensitivity of Chengdu city's young people is widespread. Acid and incorrect toothbrushing methods can cause dentine hypersensitivity. The correct protective measure to dentine hypersensitivity should be publicized.</p>

Comparison of the painless effect of four anesthetic methods during pulpal treatment / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To compare the painless effect of four anesthetic methods during opening pulp cavity and undergoing pulpectomy for acute or chronic pulpitis.</p><p><b>METHODS</b>80 teeth of 80 patients were randomly allocated into four groups. Each group had 20 teeth. Anesthetic methods applied four different groups included block anesthesia of nerve, supraperiosteal infiltration, periodontal membrane injection and intrapulpal injection. Anesthesia doses were recorded and the pierced points, the zones of pain, the time of anesthesia action, the time of anesthesia persistence and the degrees of anesthesia were evaluated with four levels synthetic evaluation standard of anesthesia.</p><p><b>RESULTS</b>Compared with periodontal membrane injection and intrapulpal injection, block anesthesia of nerve and supraperiosteal infiltration had the later time of anesthesia action and the longer time of anesthesia persistence (P<0.05). In four anesthetic methods, block anesthesia of nerve had the best painless effect (P<0.05).</p><p><b>CONCLUSION</b>Four anesthetic methods have their own superiorities, and we should select proper anesthetic methods in clinical work.</p>