Study on biocompatibility of hydroxyapatite/high density polyethylene (HA/HDPE) nano-composites artificial ossicle / 生物医学工程学杂志

This study was aimed to evaluate the biocompatibility of Hydroxyapatite/High density polyethylene (HA/ HDPE) nano-composites artificial ossicle. The percentage of S-period cells were detected by flow cytometry after L929 cells being incubated with extraction of the HA/HDPE nano-composites; the titanium materials for clinical application served as the contrast. In addition, both materials were implanted in animals and the histopathological evaluations were conducted. There were no statistically significant differences between the two groups (P >0.05). The results demonstrated that the HA/HDPE nano-composite artificial ossicle made by our laboratory is of a good biocompatibility and clinical application outlook.

Study on biocompatibility of MIM 316L stainless steel / 生物医学工程学杂志

This study was aimed to evaluate the biocompatibility of metal powder injection molding (MIM) 316L stainless steel. The percentage of S-period cells was detected by flow cytometry after L929 cells being incubated with extraction of MIM 316L stainless steel, and titanium implant materials for clinical application were used as control. In addition, both materials were implanted in animals and the histopathological evaluations were carried out. The statistical analyses show that there are no significant differences between the two groups (P > 0.05), which demonstrate that MIM 316L stainless steel has good biocompatibility.

Hydroxyapatite nanoparticles: a novel material of gene carrier / 生物医学工程学杂志

Hydroxyapatite nanoparticles were prepared in low Ca/P ratio by a kind of electrodeposition-hydrothermal process. The suspension of nanoparticles was cultured with SGC-7901 cells; metabolically active cells were evaluated by MTT analysis. Cells grew well and the nanoparticles in the concentration range of 10-100 microg/ml had no adverse effect on the cell viability. The results show that the nanoparticles have excellent biocompatibility with cells. Agrose gel electrophoresis analysis demonstrated that the nanoparticles had the potential to adsorb EGFP-N1 at the pH ranging between 2 to 7. Nanoparticle-DNA complex could transfer EGFP-N1 into the SGC-7901 cells, and the confocal microscopy analysis revealed that the cells with green fluorescence showed the efficiency of nanoparticle uptake to be about 80% of the efficiency of the Lipofectmine TM 2 000 uptake. In vivo, nanoparticles and DNA-nanoparticle complex were injected into mice respectively via tail-vein, and the mice grew well in two weeks. The liver, kidney, and brain of the mice were sampled and detected with electron microscopy, and all of these exhibited biodistribution of nanoparticles. This study demonstrates that Hydroxyapatite nanoparticles could be used as gene carriers.