Comparison of culture and PCR methods for diagnosis of vaginal infection due to Mycoplasma Hominis

Background and Objective: Mycoplasma Hominis is the smallest pathogenic bacteria, with no cell wall and free living organisms. It grows slowly and the conventional clinical microbiology techniques can not be applied due to difficulties in cultivation in particular slow growth incubation. This study was done to compare the culture and PCR methods for diagnosis of vaginal infection due to Mycoplasma Hominis

Methods: This laboratory test evaluation study was done on 150 patients with bacterial vaginosis and 50 healthy people with no infection as control, whom refereed to Imam Khomeini and Imam Zaman Hospitals in Tehran. Samples were collected in PPLO culture for growth and PBS to perform PCR method

Results: 35.3% and 76% of patients were positive using culture and PCR methods, respectively. Using PCR method 8% of control subjects was positive. There was no significant association between PCR method with abortion, place of residence and also level of educations. There was a significant association between the age [P<0.05], times of changing under wear cloths [P<0.05] and parity [P<0.05]

Conclusion: PCR method is a more reliable technique to detect the vaginal infection due to Mycoplasma Hominis compared to culturing

Association of C/T polymorphism in 3'UTR of E-cadherin gene with ovarian cancer risk

Background and Objective: Ovarian cancer is the second most common gynecological malignancy. One of the most important genes in Wnt signaling pathway is E-cadherin [CDH1], which is involved in epithelial cell-cell interaction and plays an important role in the establishment and maintenance of intercellular adhesion, cell polarity and tissue architecture. E-cadherin codes a group of connector proteins which caused to intercellular adhesion. It has an important role in adhesion of blastomere and ability to bind fetal tissues. Nucleotide change in the coding region of this gene may lead to develop ovarian cancer. This study was conducted to evaluate the association of +54C/T [Rs 1801026] 3 UTR of E-cadherin gene polymorphism with ovarian cancer risk

Methods: This case-control study was done on 100 tissue samples of patients with ovarian cancer as cases and 100 age-matched healthy women as control in Imam Khomeini Hospital, Tehran, Iran. The E-cadherin gene polymorphism was determined by using the PCR-RFLP method

Results: There was no association between CT [95% CI: 0.81-4.31; OR=1.87; P<0.14] and TT [95% CI: 0.73-2.38; OR=1.44; P<0.29] genotypes and ovarian cancer. No association was found between genotypes with grade and stage of cancer

Conclusion: There is no correlation between +54C/T [Rsl801026] 3 UTR of E-cadherin gene polymorphism with ovarian cancer