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Braz. j. microbiol ; 49(4): 909-913, Oct.-Dec. 2018. tab
Article Dans Anglais | LILACS | ID: biblio-974301

Résumé

ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.


Sujets)
Humains , Mâle , Femelle , Adulte , Tuberculose pulmonaire/diagnostic , Réaction de polymérisation en chaîne/méthodes , Réaction de polymérisation en chaine en temps réel/méthodes , Microscopie/méthodes , Mycobacterium tuberculosis/isolement et purification , Pakistan , Tuberculose pulmonaire/microbiologie , Adulte d'âge moyen , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/génétique
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