Antimicrobial activity for excretion and secretion of the greenbottle fly larvae lucilia sericata [meigen] [diptera: calliphoridae]

Sterile larval excretion/secretion [ES] exhibited antibacterial activity against some species of bacteria. They were shown to inhibit the growth of Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis Gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae and Fungi Geotricum candidum and Aspergillus fumigatus thus exhibited limited inhibitory effect towards Gram-positive bacteria Streptococcus pyogenes and Staphylococcus epidermidis and Gram-negative Proteous vulgaris and Fungi Syncephalastrum racemosum, Candida albicans, that effect was slowed down when challenged with secretion on a solid media but no zone of complete inhibition was detected. Growth inhibiting activity was determined in liquid growth media using the Gram-positive, Gram-negative bacterial and fungal strains as indicator organisms

using of lucilia cuprina maggots in the treatment of diabetic foot wounds

In the present study, Lucilia cuprina maggots were used for the treatment of diabetic foot wounds. An artificial wound was made in diabetic foot of rabbit. The maggots were sterilized arid put directly on the wound after dressing the wound without using any antibiotics. Several cycles of maggots were put on the wound. The present results showed that the treatment of the diabetic foot was observed after 13 days. After this period the wound was completely healed and become free of microbial contamination. The new tissues were observed to close the wound

Repellent effect of lagenaria siceraria extracts against culex pipiens

Ethanolic, acetone and petroleum ether extracts from leaves and stems of Lagenaria siceraria [Cucurbitaceae] were screened for their repellency effect against Culex pipiens L. mosquito. The repellent action of the present plant extracts were varied depending on the plant parts and the dose of extract. The petroleum ether extract of leaves showed the same repellency percent [100%] of commercial formulation, N. N.'diethyl toulamide [DEBT] at the higher dose [3.33 mg/cm[2]], while petroleum ether extract from stems exhibiting the repellent action [89.6%] at the same dose, respectively. Ethanolic extracts of leaves and stems exhibited the lowest repellent activity as it recorded [81.3% and 69.1%] at [6.67 mg/cm[2]], respectively. Results of this study may contribute to design an alternative way to control mosquitoes currently based on applications of synthetic insecticides. These extracts could be developed commercially as an effective personal protection measure against mosquito bites and thus to control diseases caused by mosquito-borne pathogens

Effects of midgut bacteria and two protease inhibitors on the reproductive potential and midgut enzymes of culex pipiens infected with wuchereria bancrofti

Laboratory investigations were carried out to asses the effect of some proteases inhibitors on the reproductive potential of Culex pipiens females resulted from larvae treated with different protease inhibitors. The fecundity and engorgement of symbiotic and aposymbiotic C. pipiens females were significantly reduced. The blood meal digestion period increased significantly. On the other hand, enzyme band with molecular weight of 40 KDa which may be cysteine protease was detected in untreated symbiotic and aposymbiotic female midguts. The results may explain that the absence of this enzyme bands in treated female midguts may be due to the inhibition caused by [E-64] a cysteine protease inhibitor

Effects of midgut bacteria and two protease inhibitors on the transmission of wuchereria bancrofti by the mosquito vector, culex pipiens

Laboratory investigations were carried out to study the effect of two protease inhibitors on the transmission of W. bancrofti filarial by Culex pipiens and to study the susceptibility interaction between filaria and protease inhibitors. The results obtained revealed that, infection rates were variable among untreated and treated symbiotic and aposymbiotic Cx. pipiens females resulted from third instar larvae treated with E-64 and EDTA. The survival rate was variable among untreated and treated symbiotic and aposymbiotic females resulted from third instar larvae treated with E-64 and EDTA. Protease inhibitor [E-64] caused inhibition of the parasite development and transmission by means of ceasing catalytic activity- responsible for parasite migration-caused by parasitic larval stages inside the mosquito vector

Mosquito species diversity and abundance in relation to riceland agroecosystem and filarial infection in Kafr El-Sheikh Governorate, Egypt

The present work studied the mosquitoes abundance, identification, distribution and density in three villages [rural area] and one city [urban area] in Kafr El-Sheikh Governorate namely; Kebreet, Minyat Al-Ashraaf, El-Salmia and Fowa city, respectively during the rice cultivation season in relation to filaria from June to Oct. 2009. A total of 11381 mosquitoes larvae belonging to four genera and 8 species were collected. Of which 3525 [31.0%] in Minyat Al-Ashraaf followed by 3339 [29.3%] in Kebreet, 3331 [29.3%] in El-Salmia villages compared with 1186 [10.4%] in Fowa city. The five most common species collected during this study were Culex pipiens [39.2%], Cx. antennatus [27.3%], Cx. univittatus [15.8%], Anopheles pharoensis [10.4%], and An. coustani[3.8%]. The mosquito species diversity [H] and evenness [EH] in the [rice cultivated areas] Minyat Al-Ashraf, Kebreet and El-Salmia villages [H = 1.286, EH= 0.829; H= 1.227, EH= 0.742; H= 1.110, EH=0.882; respectively] were much higher than in the Fowa city [non rice cultivated area] [H= 0.718, EH= 0.608]. On the other hand, the highest diversity and density of adult mosquitoes species obtained from Minyat Al-Ashraaf were 5 species and [33.8%], followed by Kebreet 5 species and [31.6%], El-Salmia 4 species and [24.5%], respectively compared with 3 species and [10.1%] in Fowa city. Cx. pipiens adults were the predominant species, in all filarial indicator areas [68.1, 53.4, 40.8 and 20.8 mosquitoes/room] in Minyat Al-Ashraaf, Kebreet, El-Salmia villages and Fowa city, respectively. Cx. pipiens was the only species to cany infective larvae as well as other stages, while Cx. antennatus carried immature stages only [not infective]. Filarial larvae in Cx. pipiens and Cx. antennatus were found only in Minyat Al-Ashraaf and Kebreet villages. It is inferred from the data that different levels of habitat with regard to rice cultivation have different effects on mosquito diversity and abundance. Also, our study revealed that filarial vectors Cx pipiens and Cx. antennatus had a wide distribution and high relative density especially in irrigated rice regions and hence its role in disease transmission in Kafr El-Sheikh region needs further investigation