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Journal of Veterinary Science ; : 17-23, 2015.
Article Dans Anglais | WPRIM | ID: wpr-206917

Résumé

Butylated hydroxyanisole (BHA) is a synthetic phenolic compound consisting of a mixture of two isomeric organic compounds: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. We examined the effect of BHA against hydrogen peroxide (H2O2)-induced apoptosis in primary cultured mouse hepatocytes. Cell viability was significantly decreased by H2O2 in a dose-dependent manner. Additionally, H2O2 treatment increased Bax, decreased Bcl-2, and promoted PARP-1 cleavage in a dose-dependent manner. Pretreatment with BHA before exposure to H2O2 significantly attenuated the H2O2-induced decrease of cell viability. H2O2 exposure resulted in an increase of intracellular reactive oxygen species (ROS) generation that was significantly inhibited by pretreatment with BHA or N-acetyl-cysteine (NAC, an ROS scavenger). H2O2-induced decrease of cell viability was also attenuated by pretreatment with BHA and NAC. Furthermore, H2O2-induced increase of Bax, decrease of Bcl-2, and PARP-1 cleavage was also inhibited by BHA. Taken together, results of this investigation demonstrated that BHA protects primary cultured mouse hepatocytes against H2O2-induced apoptosis by inhibiting ROS generation.


Sujets)
Animaux , Mâle , Souris , Apoptose/effets des médicaments et des substances chimiques , Hydroxyanisole butylé/composition chimique , Survie cellulaire/effets des médicaments et des substances chimiques , Cellules cultivées , Hépatocytes/effets des médicaments et des substances chimiques , Peroxyde d'hydrogène/toxicité , Souris de lignée ICR , Structure moléculaire
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