Résumé
Liquiritigenin (LQ) is a flavonoid that can be isolated from Glycyrrhiza radix. It is frequently used as a tranditional oriental medicine herbal treatment for swelling and injury and for detoxification. However, the effects of LQ on cognitive function have not been fully explored. In this study, we evaluated the memory-enhancing effects of LQ and the underlying mechanisms with a focus on the N-methyl-D-aspartic acid receptor (NMDAR) in mice. Learning and memory ability were evaluated with the Y-maze and passive avoidance tests following administration of LQ. In addition, the expression of NMDAR subunits 1, 2A, and 2B; postsynaptic density-95 (PSD-95); phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII); phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2); and phosphorylation of cAMP response element binding (CREB) proteins were examined by Western blot. In vivo, we found that treatment with LQ significantly improved memory performance in both behavioral tests. In vitro, LQ significantly increased NMDARs in the hippocampus. Furthermore, LQ significantly increased PSD-95 expression as well as CaMKII, ERK, and CREB phosphorylation in the hippocampus. Taken together, our results suggest that LQ has cognition enhancing activities and that these effects are mediated, in part, by activation of the NMDAR and CREB signaling pathways.
Sujets)
Animaux , Souris , Échelle d'évaluation du comportement , Technique de Western , Calcium-Calmodulin-Dependent Protein Kinase Type 2 , Cognition , Glycyrrhiza , Hippocampe , Techniques in vitro , Apprentissage , Médecine traditionnelle d'Asie orientale , Mémoire , N-Méthyl-aspartate , Phosphorylation , Phosphotransferases , Protein kinases , Récepteurs du N-méthyl-D-aspartate , Éléments de réponseRésumé
No abstract available.
Résumé
Recently new tumor marker, MG7-Ag has been introduced for screening of gastric cancer. In this study MG7-Ag and CEA in sera of 50 normal healthy Koreans and 48 patients with gastric cancer group were determined to elucidate the clinical usefulness for gastric cancer screening. Commercial Enzyme Immuno Assay kits were used for analysis of above two markers. Sensitivity of MG7-Ag and CEA in patient group were found to be 43.8% and 41.7%, respectively. When the two markers were used combind, sensitivity increased to 62.5%. The concentration of MG7-Ag in patients (9.2+11.7 U/mL) was five times higher than normal control group (1.81+1.63 U/mL). CEA was 31.2+46.5 ng/mL in patients and 2.24+0.98 ng/mL (MeanSD) in normal control group. From this results, combination assay of MG7-Ag and CEA is more useful in clinical laboratories for screening gastric cancer than using one marker.
Sujets)
Humains , Dépistage de masse , Tumeurs de l'estomacRésumé
No abstract available.
Résumé
Removal of blunt esophageal foreign bodies using Foley catheter under a fluoroscopic guidance is a well-recognized procedure. However, since this procedure is rather cumbersome and uncomfortable to the patient, the authors tried to find an easier and more convenient modified technique. For 10 patients with esophageal foreign body, we tried the method to the patients who is lying in the right lateral decubitus position and 3 assistants hold head. arms, trunk and legs of the patients without tilting the table and without using immobilizer. Foley catheter is inserted through nostril, nasal cavity and pharynx to esophagus. In order to identify the Foley catheter in esophagus, 0.025 inch short wire was inserted in the Foley catheter. The balloon of a Foley catheter was inflated by 10cc of air, and the syringe was kept attached to the Foley catheter during the procedure. After passage of the foreign body through the upper esophageal sphincter, the balloon was deflated immediately and the foreign body was removed through the mouth. We successfully removed in removing all the blunt esophageal foreign body with ease. This modified method is also fast, safe and efficient.
Sujets)
Humains , Bras , Cathéters , Tromperie , Sphincter supérieur de l'oesophage , Oesophage , Corps étrangers , Tête , Jambe , Méthodes , Bouche , Fosse nasale , Pharynx , SeringuesRésumé
MRI (magnetic resonance imaging ) had been the most up-to-dated modality in evaluating white matter disease in recent years, whereas US (ultrasonogram) has been used extensively in diagnosis of neonatal PVL(periventricular leukomalacia) conventionally. We evaluated the diagnostic value of MRI by reviewing the MR findings and correlation of MR and US of PVL in II preterm infants. Evaluation criteria were MR signal intensity and discrimination of PVL on each pulse sequences land comparision between MR and US findings performed simultaneously, on the extent of PVL, size of the largest cyst and detectability of hemorrhagic lesion. MR findings of 11 cases of PVL were of low signal patterns on T1WI (T1weighted image) in 7, low signal patterns of PDWI (proton density weighted image) in 9, iso signal patterns on T2WI(T2weighted image) in 8 and low signal patterns on STIR(short time inversion recovery) in 7 cases. The lesions of 11 PVL were well discriminated in all 11 cases of T1WI, 7 cases of STR, 5 case of PDWE and 2 cases of T2WI. The lateral ventricle was diffuse dilated (n=1) and focally dilated (n=3) in atrial area. In the comparative study, MR presented more extensive lesions in 7 cases, larger cysts in 6 cases out of 7 PVL and more definitive hemorrhage in 3 cases out of 4 cases than sonography. In conclusion MR was more valuable than sonography in evaluating the extent of lesions, size of the largest cystic lesion and detetion of hemorrhage. TIWI and STIR images were more useful in detection of PVL than T2WI and PDWI.
Sujets)
Humains , Nouveau-né , Diagnostic , , Hémorragie , Prématuré , Ventricules latéraux , Leucoencéphalopathies , Imagerie par résonance magnétiqueRésumé
Intracranial nerve sheath tumors unrelated to the cranial nerve roots are extremely rare, and the origin of the tumors are debatable. We report a case of pathologically-proven neurofibroma inside the lateral ventricle. A 49-year-old man presented with headache of 6 months duration, urinary incontinence, visual disturbance and right hemiplegia. Brain CT scan showed a well defined isodense mass with homogenous contrast enhancement and marginal calcification. At surgery the tumor was found to be a 4cm-sized lobulated mass attached only to the choroid plexus. Histologically, the tumor masses consisted of fasciculating bundles of wavy spindle cells, with a considerable collagen laydown.