Stability indicating methods for the determination of aescin in phytopharmaceuticals

Two methods are presented for the determination of aescin in the presence of its degradates. Aescin is a saponin glycoside undergoes acid hydrolysis and the aglycon is produced. The first method is based on determination of aescin by HPLC with UV detection at 230 nm. The mobile phase used is acetonitrile: water[90:10v/v] and the pH was adjusted with orthophosphoric acid at pH 3.5. linearity range is 100-700 microg/ml. The second method is a densitometric one based on the determination of aescin in the presence of its degradates at 215 nm using the mobile phase, butanol: acetic acid: water [4:125 v/v]. linearity range is 2-14 micro g/spot. The proposed methods were applied for the determination of aescin in phytopharmaceuticalst. The validity of the described methods was assessed by applying the standard addition technique. Statistical analysis of the results has been carried out recording high accuracy and precision. The suggested methods could be used for the determination of aescin, both in pure form and dosage forms, as well as in the presence of its degradates. No chromatographic interference from excipients was found

colorimetric method for analysis of phenolphthalein

A colorimetric procedure for the determination of phenolphthalein based upon its condensation with 4-aminophenazone in presence of an alkaline oxidizing agent and measuring the absorbance at 460 nm, is proposed. The procedure determines 0.08-0.6 mg/25 ml with mean recovery of 99.54 +/- 0.64%. The method is compared by the British Pharmacopoeia [1980] method and is applied to the determination of phenolphthalein in pharmaceutical preparations. The proposed procedure is not interferred with by other compound usually formulated with phenolphthalein