Effects of different concentrations of calcium on motility pattern of ovine epidiymal sperm in modified bo

To evaluate the effects of calcium on motility patterns of bovine epididymal sperm and to determine the effective calcium concentration, ovine testicles [5 for each experiment day] were picked up in a local slaughterhouse and transported to the laboratory in a cool container [filled wich ice].In laboratory,caudal epididymis was incised and sperm cells were put into Petri dishes containing modified BO medium which were kept in37?C incubator. To separate sperm from epididymal particles swim up method was performed into BO medium containing 0.56, 1.125 and 2025 mM calcium. Motility pattern were assessed at the start of experiment [0h] and2,4and 6h post incubation using Computer Assisted Sperm Analyzing [CASA] and different motility parameters were assessed The proportions sperm motility were analyzed using a mathematical model that included fixed effect due to treatment [calcium concentrations] and residual error. When the analysis revealed a significant effect, the values were compared by duncan's multiple range test. The results showed that no significanet different was observed in the start of experiment [no fast effect].Two h post incubation the proportion of class A spermatozoa [fast moving ones], Curvilinear velocity [VCL], Straight line velocity [VSL], Average path velocity [VAP] and Strightness [STR]in 0.56 mM calcium were significantly higher than those in 2.25 mM calcium [p < 0.05]. Four h post incubation, the proportion of class A and class C spermatozoa [not moving ones], class A+B [alive sperm cells], VCL, VSL,VAP,Linearity and STR in 0.56 mM calcium were significantly higher than those in 2025 nM calcium [p < 0.05]. Six h post incubation only STR was significantly different between the concentrations of 0.56 and 2025 mM calcium. Howevent, concentrations of calcium.The results of the present study indicate the lower calcium concentrations may support a higher sperm motility proportion in BO medium

[Comparative studies of the electrocardiographic parameters, mean electrical axes [MEA] and cardiac index[RV/TV]in two groups of normal and experimentally ascitic broilers [using cold stress as a model]]

To evaluate the electrocardigraphic changes in the experimentally induced ascitis in broiler chickens using cold stress as a model. Experimental study.A total of 300 day-old male Ross 308 broiler chickes.Chickenswere randomly divided into two equal groups, [control and test], chicks were reared for six weeks. Gradually Temperature was gradually decreased in the test group to 30% of the standard program from 2 nd week of the rearing period. At the end of each week, 5 chicks from each group were selected and electrocardiographic recordings were performed. Also after autopsy right ventricle / total ventricular weight was measured and recorded. t- student Test and Bivariate correlation. There were significant elevation of the S wave amplitudes in 4th week [leads II, III, aVR]and 6th week [lead III] .S wave had a significant correlation with RV/TV in 4th week[lead II] and 5th week [leadsII, aVF] in the test group, too. There were also significant increase in the T wave amplitudes in 4[th] and 5[th] weeks [leadsII, aVF]and 6[th] week[leads aVF, aVR] in the test group.In this study, R waves did not show a significant variation with the exception of 4[th] week[lead II]. There were an elevation of MEA in test groups [with a right-cranial direction] but it just was significant in 4th week compared with control group. Electrocardiograms can be used effectively to evaluate the development of ascites syndrome in broiler chickens. The increase in the amplitude of S and T waves could be considered as the sign of ventricular hypertrophy in ascites syndrome resulted from cold condition