Nonmedication treatment of attention deficit hyperactivity disorder in children / 中国学校卫生

Abstract@#Attention deficit hyperactivity disorder (ADHD) is a common childhood psycho behavioral disorder, the exact cause of which is currently unclear. It is generally believed that its incidence is a combination of biological, environmental, and psychological factors. In China at present, the common intervention measures for this disorder are mainly drug treatments, and non medication treatment including EEG Biofeedback environmental Modifications and Self regulation, is relatively inadequate. Non medication treatment is generally targeted at the social function defects associated with this disease; its efficacy is longer lasting and it is safer. This article analyzes the principles, methods, efficacy, shortcomings, and prospects of the non medication treatment of ADHD.

Synergistic Effect of Hyperthermia and Neferine on Reverse Multidrug Resistance in Adriamycin-resistant SGC7901/ADM Gastric Cancer Cells / 华中科技大学学报（医学）（英德文版）

Multidrug resistance (MDR) plays a major obstacle to successful gastric cancer chemotherapy.The purpose of this study was to investigate the MDR reversal effect and mechanisms of hyperthermia in combination with neferine (Nef) in adriamycin (ADM) resistant human SGC7901/ADM gastric cancer cells.The MDR cells were heated at 42℃ and 45℃ for 30 min alone or combined with 10 μg/mL Nef.The cytotoxic effect of ADM was evaluated by MTT assay.Cellular plasma membrane lipid fluidity was detected by fluorescence polarization technique.Intracellular accumulation of ADM was monitored with high performance liquid chromatography.Mdr-1 mRNA,P-glycoprotein (P-gp),γH2AX expression and γH2AX foci formation were determined by real-time PCR,Western blot and immunocytochemical staining respectively.It was found that different heating methods induced different cytotoxic effects.Water submerged hyperthermia had the strongest cytotoxicity of ADM and Nef combined with hyperthermia had a synergistic cytotoxicity of ADM in the MDR cells.The water submerged hyperthermia increased the cell membrane fluidity.Both water submerged hyperthermia and Nef increased the intracellular accumulation of ADM.The water submerged hyperthermia and Nef down-regulated the expression of mdr-1 mRNA and P-gp.The water submerged hyperthermia could damage DNA and increase the γH2AX expression of SGC7901/ADM cells.The higher temperature was,the worse effect was.Our results show that combined treatment of hyperthermia with Nef can synergistically reverse MDR in human SGC7901/ADM gastric cancer cells.