Résumé
Objective To investigate the function of autophagy in the process of PM2.5-induced apoptosis. Methods PM2.5 was obtained from Zhanjiang in 2016. Human lung adenocarcinoma cells H441 were treated with PM2.5 at different concentrations for different times. Cell proliferation was analyzed by MTT assay; Cell apoptosis was assessed by PI and Annexin V double staining and TUNEL assay. The expression of autophagy marker LC3Ⅱ, AKT and P-AKT protein was examined by Western blot ( WB). H441 cells were treated with PM2.5 following treatment with rapamycin or 3-MA. Cell viability was evaluated by trypan blue staining. Results Compared with the control group, cell proliferation was significantly inhibited by PM2.5 at concentration of 100 μg/mL or more for 24 and 48 h. With the increase of PM2.5 concentration, the cells apoptotic rate significantly increased, the protein ex-pression of LC3Ⅱwas increased as well as the P-AKT was decreased; and the protein expression of LC3Ⅱwas in-creased significantly after AKT inhibitor treatment. Moreover, rapamycin decreased PM2.5-induced cell apoptosis, and 3-MA can promote PM2.5-induced cell apoptosis. Conclusions In H441 cells, PM2.5 activates autophagy by inhibiting activation of AKT pathway, and cell autophagy can mitigate PM2.5-induced apoptosis.
Résumé
Objective To observe the preemptive analgesia effect of parecoxib sodium in patients undergoing laparoscopic cervical carcinoma radical resection.Methods Seventy patients undergoing laparoscopic cervical carcinoma radical resection were randomly divided into 2 groups with 35 cases each. Parecoxib sodium 40 mg was injected intravenously 10 min before operation and repeatedly given every 12 h. Equal volume physiological saline was given at same time in the control group. Two groups received postoperative PCIA with morphine. The numerical rating scale （NRS） was used to rate pain intensity at following time points：immediately after extubation,2,6,12,18,and 24 h after operation. Twenty-four hour morphine consumption and side effects were recorded.Results The NRS rating of pain at each time point in the parecoxib group was significantly lower than that of the control group （P0.05）,and the total morphine consumption （10.4±7.6）mg was less than the control （17.7±8.9）mg （P0.05）; correspondingly,the incidences of nausea,vomiting and drowsiness were less,and the number of patients left bed for activity was increased in the parecoxib group than those in the control one （P0.05）. Conclusion Preoperative parecoxib sodium 40 mg can improve the analgesic effect of PCIA with morphine,and reduce morphine consumption and the incidences of side effects.