The effects of enamel matrix proteins on biological characteristics of human dental follicle cells in vitro / 实用口腔医学杂志

Objective:To investigate the effects of enamel matrix proteins(EMPs) on proliferation and alkaline phosphotase (ALP) activity of human dental follicle cells(HDFCs) in vitro. Methods:HDFCs were dissected and cultured by digestion with bacterial collagenase. MTT method and enzyme kinetics method were used to examine the proliferation and ALP activity of HDFCs treated with EMPs.Results:EMPs at 25~200 mg/L increased the proliferation of HDFCs and EMPs at 100 mg/L showed the strongest effects in 7-day-culture.EMPs at 100 mg/L increased ALP activity of HDFCs in 3- and 7-day cultures.Conclusion:EMPs may stimulate the proliferation and the ALP activity of HDFCs.