Cloning of a B10 DNA puff sequence developmentally amplified and expressed in the salivary gland of Bradysia hygida

A recombinant clone carrying a 2-kb fragment was isolated from a mini-library of the B10 DNA puff of Bradysia hygida. This fragment was amplified in the salivary gland during the period of DNA puff formation. Amplification started when DNA puff anlage was formed and continued to increase, reaching a maximum of abouth 10-fold 28 h later. Northern blot hybridization experiments showed that this 2-kb fragment was complementary to two RNA species of about 1.3 kb and 1.1 kb, which are developmentally regulated in the salivary gland. Maximum amounts of these messages were present when the B10 puff is fully expanded

Hemoglobin H disease caused by two gene deletions

1. A white Brazilian woman not of Asian origin was found to have Hb H disease of moderate severity. 2. Gene mapping demonstrated that the disease was caused by the association of two abnormal alfa-globin gene clusters on chromosome 16: one with a deletion which removed the two function alfa genes and the other carryng the 3.7-Kb rightward deletion, which leaves functional alfa gene. 3. These data ilustrate the necessity for systematic molecular approaches to the diagnosis of this heterogeneous group of diseases