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Braz. j. med. biol. res ; 27(9): 2191-5, Sept. 1994. graf
Article Dans Anglais | LILACS | ID: lil-144472

Résumé

The mechanism by which heparin and antithrombotic agents, including a cyclic octaphenolsufonic acid (compound Y), stimulate the synthesis of an antithrombotic heparan sulfate by endothelial cells in culture was investigated. Compound Y increases the amount of heparan sulfate from the cell surface and secreted to the endothelial cell receptors at a concentration of 0.16µM for heparin and 2.7µM for compound Y. The kinetic binding constants (Ks) for compound Y and heparin were 1,333 nM and 42 nM, respectively. It was also shown that both compounds bind to the same receptors. The Scatchard plots indicated that 1,319 nmoles compound Y and 35 nmoles heparin bound per microgram cell protein, indicating that 40-fold more molecules of compound Y bound to the receptors when compared to heparin. No significant internalization of the compounds was observed


Sujets)
Cochons d'Inde , Lapins , Animaux , Endothélium vasculaire/métabolisme , Héparine/pharmacologie , Héparitine sulfate/biosynthèse , Protéoglycanes/biosynthèse , Lignée cellulaire , Cellules cultivées , Endothélium vasculaire/cytologie , Température élevée , Liaison aux protéines , Facteurs temps
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