RÉSUMÉ
Objective:To construct CD106-targeted RNAi lentiviral vector plasmids. Methods:4 targets aimed at CD106(Target 1, 2, 3, 4)were designed. Oligo-DNA fragment containing short hairpin frame was synthesized and reannealed, and then cloned into lentivi-ral expression vector. PCR and sequencing analysis were made for verifying the positive clones. The virus packaging plasmids were trans-fected into 293T cells to harvest siRNA lentivirus. After infection in HN12 cells, Real-time PCR and western blot were performed to de-termine the expressing level of CD106. Results:PCR and sequencing revealed that siRNA plasmids was correctly constructed. Virus with a titer of 1 × 109 TU/ml was successfully packaged at least. CD106 expression in HN12 cells could be knockdown by virus infection sig-nifically, compared with negative control lentivirus. Conclusion: The recombinant lentiviral siRNA expressing vector targeting human CD106 gene has been successfully constructed and packaged. CD106 gene in cells may be down-regulated by lentiviral siRNA.
RÉSUMÉ
Objective: To investigate the role of vascular cell adhesion molecule-1(VCAM-1)on macrophage infiltration and recruitment in oral squamous cell carcinoma(OSCC). Methods:After detecting the expression of VCAM-1 and macrophage infiltration in OSCC by immunohistochemistry, light microscope was used for counting macrophages. Results:The expression level of VCAM-1 (64.58%)and macrophage numbers(81.04?12.00) was significantly higher in OSCC than those in normal oral mucosa. The expression of VCAM-1 in OSCC was not only related to the tumor infiltration and metastasis in OSCC(P