Antibiotic susceptibility profile of Helicobacter pylori isolated from the dyspepsia patients in Tehran, Iran

Helicobacter pylori is an important pathogen for gastroduodenal diseases. Infection with H. pylori can be limited by regimens of multiple antimicrobial agents. However, antibiotic resistance is a leading cause of treatment failure. The aim of this study has been to determine the resistance patterns of H. pylori strains isolated from gastric biopsies of patients with dyspepsia by agar dilution method, in Tehran, Iran. H. pylori isolates from patients with gastrointestinal diseases were evaluated for susceptibility testing by agar dilution method. Susceptibility testing was performed to commonly used antibiotics including clarithromycin, tetracycline, amoxicillin, metronidazole and ciprofloxacin. Among 92 patients with dyspepsia, H. pylori strains were isolated from 42 patients. Seventeen [40.5%] of the isolates were resistant to metronidazole [MICs >/= 8 microg/1], whereas one isolate [2.4%] was resistant to amoxicillin [MICs

Increase in prevalence of vancomycin resistant isolates of Enterococcous faecium at Labbafinejad hospital

Vancomycin resistant isolates of Enterococcous faecium [VRE] have previously been reported from Tehran Hospitals. However, little data were available on the genetic heterogeneity of VRE isolates among the Iranian population. Therefore the emergence of infections with the new clones of VRE needs to be investigated. The drug resistance surveillance program at Labbafinejad hospital has to be continued. Overall, 103 non-replicative isolates of enterococci grown from urine samples in the first quarter of 2005 were screened for their susceptibilities to different antibiotics. Ribotyping was then used to genetically characterize the isolates of VRE. Using disk diffusion method, all isolates were found susceptible to linezolid. Resistance to high level concentration of gentamicin was detected in 65.7% of isolates. All isolates of F. faecalis [n=86] were susceptible to vaneomycin. Conversely, over 70% of E. faecium isolates [n=12] showed resistance to this glycopeptide. The VRE isolates recovered from patients in 2005 were heterogeneous comparing with those of 2000. Conventional bacteriology confirmed the increase in the rate of VRE. It appears that a variety of new VRE clones have arisen recently at different wards of this hospital as determined by ribotyping