Empiema subdural recidivante secundário à sinusite bacteriana frontal em um adolescente imunocompetente: relato de caso / Relapsing subdural empyema secondary to frontal bacterial sinusitis in an immunocompetent adolescent: case report

A sinusite é uma causa rara de infecção intracraniana, sendo responsável por 2,4% dos casos em pacientes jovens, e tem como a complicação intracraniana mais frequente o empiema subdural (ESD). Descrevemos um caso raro de um adolescente imunocompetente de 16 anos que evoluiu com confusão mental, rebaixamento do nível de consciência, anisocoria, hemiparesia à direita, afasia e febre. Tomografa computadorizada confirmou ESD, e paciente foi submetido à avaliação laboratorial e abordagem clínico-cirúrgica para tratamento do quadro.

Sinusitis is a rare cause of intracranial infection, accounting for 2.4% of cases in young patients. The most frequent intracranial complication is subdural empyema (SDE). We describe a rare case of a 16-year-old immunocompetent adolescent who developed mental confusion, lowered consciousness, anisocoria, right hemiparesis, aphasia, and fever. Computed tomography confirmed SDE, and the patient underwent laboratory evaluation and clinical-surgical approach for treatment of the condition.

Acid phosphatase activity in ungerminated conidia from Colletotrichum graminicola as determined by spectrophotometric and cytochemical methods

The acid phosphatase in ungerminated conidia from Colletotrichum graminicola, a corn pathogen, was investigated using spectrophotometric and cytochemical methods. Acid phosphatase activity was studied in a homogenate obtained by fragmentation of ungerminated conidia. With p-nitrophenylphosphate as substrate, the apparent Vmax and Km were 1.000 nmol p-nitrophenol/mg of protein/min and 0.631 mM, respectively. The pH and temperature optima were 5.5 and 60 graus Celsius, respectively. A cytochemical ultrastructural assay showed deposition of the reaction product inside vacuoles but not extracellularly on the cell surface. The permeabilization of conidia with Triton X-100 increased acid phosphatase activity eight fold. Compared to other procedures, our method was fast, easy to perform and gave consistent results.